News Column

Studies from Third Military Medical University Yield New Data on Sulfur Amino Acids

February 11, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Sulfur Amino Acids have been published. According to news reporting from Chongqing, People's Republic of China, by NewsRx journalists, research stated, "In this work, a novel peroxydisulfate electrochemiluminescence (ECL) strategy based on a sandwich-type immunosensor for sensitive detection of carcinoembryonic antigen (CEA) was developed. The primary antibody anti-CEA (Ab(1)) was immobilized onto Au nanopractices (AuNPs) electrodeposited onto the electrode, which have large surface area and high electrical conductivity."

The news correspondents obtained a quote from the research from Third Military Medical University, "Then, L-cysteine (L-Cys) and AuNPs functionalized multiwalled carbon nanotubes (MWCNTs) were synthesized to act as the platform for immobilization glucose oxidase (GOD), horseradish peroxidase (HRP) and secondary antibody (Ab(2)). The CEA antigen and MWCNTS-L-Cys-AuNPs@GOD-HRP-labeled Ab(2) were then successively conjugated to form sandwich-type immunocomplexes through the specific interaction between antigen and antibody. The ECL signal amplification was significantly improved due to the synergistic effect of AuNPs and L-Cys. Furthermore, when proper amounts of D-glucose were added in the detection solution, GOD catalyzed the oxidation of glucose to generate H2O2, which could be further catalyzed by HRP to generate O-2 for the signal amplification. The developed ECL immunosensor exhibited high sensitivity and specificity for the detection of CEA and responded linearly to the clinically relevant concentration of CEA from 0.02 to 80 ng mL(-1) with a low detection limit of 0.67 pg mL(-1)."

According to the news reporters, the research concluded: "The present work provided a promising technique for highly sensitive bioassays applied in clinical detection."

For more information on this research see: A novel electrochemiluminescent detection of protein biomarker using L-cysteine and in situ generating coreactant for signal amplification. Sensors and Actuators B-Chemical, 2014;192():685-690. Sensors and Actuators B-Chemical can be contacted at: Elsevier Science Sa, PO Box 564, 1001 Lausanne, Switzerland (see also Sulfur Amino Acids).

Our news journalists report that additional information may be obtained by contacting A.L. Sun, Third Military Medical University, Inst Hepatobilitary Surg, Chongqing 400038, People's Republic of China. Additional authors for this research include Q.A. Qi, X.N. Wang and P. Bie.

Keywords for this news article include: Asia, Cysteine, Chongqing, Sulfur Amino Acids, Neutral Amino Acids, Sulfhydryl Compounds, People's Republic of China

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Source: Life Science Weekly

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