By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on DNA Research are discussed in a new report. According to news originating from Athens, Greece, by NewsRx correspondents, research stated, "Leishmaniosis is a zoonose caused by protozoans of the genus Leishmania. The need for accurate diagnostic investigation of cases of leishmaniosis has rendered today the use of molecular biology techniques broadly applicable."
Our news journalists obtained a quote from the research from Biomedical Research Foundation, "However, the reliable application of these methods requires highly-specialised personnel, dedicated equipment and space. The aim of this study was the design and construction of functionalized gold nanopartides (AuNPs) that would be incorporated into an easily applicable DNA detection methodology for the identification of Leishmania spp. in clinical samples. AuNPs 20 nm in diameter were conjugated with four oligonucleotide probes, targeting kinetoplastid minicircle DNA of Leishmania spp. In the absence of complimentary DNA, AuNPs-probes precipitate under acid environment causing a change of color from red to purple, which can be detected by visual observation. In the presence of target DNA the color of the solution remains red. The specific methodology was applied to positive and negative control samples and whole blood collected from dogs with suspected canine leishmaniosis. The method's minimum detection limit was defined to 11.5 ng of target DNA per mu l of sample. Repeatability and reproducibility were 100%. Relative sensitivity and specificity referenced to PCR were calculated to 92% and 100% regarding collectively control and clinical samples."
According to the news editors, the research concluded: "The proposed approach can be considered an appealing diagnostic solution especially for screening purposes in enzootic areas, where detection of very small amounts of the targeted analyte is not top priority."
For more information on this research see: A novel non-amplification assay for the detection of Leishmania spp. in clinical samples using gold nanoparticles. Journal of Microbiological Methods, 2014;96():56-61. Journal of Microbiological Methods can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Microbiological Methods - www.elsevier.com/wps/product/cws_home/506034)
The news correspondents report that additional information may be obtained from M. Andreadou, Academy Athens, Biomed Res Fdn, Bioinformat & Med Informat Team, Athens 11527, Greece. Additional authors for this research include E. Liandris, M. Gazouli, S. Taka, M. Antoniou, G. Theodoropoulos, I. Tachtsidis, N. Goutas, D. Vlachodimitropoulos, I. Kasampalidis and J. Ikonomopoulos (see also DNA Research).
Keywords for this news article include: Athens, Greece, Europe, DNA Research, Nanoparticle, Nanotechnology, Emerging Technologies
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