By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- Data detailed on Biotechnology have been presented. According to news reporting originating in Cambridge, United Kingdom, by NewsRx journalists, research stated, "Oestrogen receptor alpha (ER alpha) is a nuclear receptor that is the driving transcription factor expressed in the majority of breast cancers. Recent studies have demonstrated that the liver receptor homolog-1 (LRH-1), another nuclear receptor, regulates breast cancer cell proliferation and promotes motility and invasion."
The news reporters obtained a quote from the research from Cambridge Research Institute, "To determine the mechanisms of LRH-1 action in breast cancer, we performed gene expression microarray analysis following RNA interference for LRH-1. Interestingly, gene ontology (GO) category enrichment analysis of LRH-1-regulated genes identified oestrogen-responsive genes as the most highly enriched GO categories. Remarkably, chromatin immunoprecipitation coupled to massively parallel sequencing (ChIP-seq) to identify genomic targets of LRH-1 showed LRH-1 binding at many ER alpha binding sites. Analysis of select binding sites confirmed regulation of ER alpha-regulated genes by LRH-1 through binding to oestrogen response elements, as exemplified by the TFF1/pS2 gene. Finally, LRH-1 overexpression stimulated ER alpha recruitment, while LRH-1 knockdown reduced ER alpha recruitment to ER alpha binding sites."
According to the news reporters, the research concluded: "Taken together, our findings establish a key role for LRH-1 in the regulation of ER alpha target genes in breast cancer cells and identify a mechanism in which co-operative binding of LRH-1 and ER alpha at oestrogen response elements controls the expression of oestrogen-responsive genes."
For more information on this research see: Co-regulated gene expression by oestrogen receptor alpha and liver receptor homolog-1 is a feature of the oestrogen response in breast cancer cells. Nucleic Acids Research, 2013;41(22):10228-10240. Nucleic Acids Research can be contacted at: Oxford Univ Press, Great Clarendon St, Oxford OX2 6DP, England. (Oxford University Press - www.oup.com/; Nucleic Acids Research - nar.oxfordjournals.org)
Our news correspondents report that additional information may be obtained by contacting C.F. Lai, Canc Res UK, Cambridge Res Inst, Li Ka Shing Center, Cambridge CB2 0RE, United Kingdom. Additional authors for this research include K.D. Flach, X. Alexi, S.P. Fox, S. Ottaviani, P.T.R. Thiruchelvam, F.J. Kyle, R.S. Thomas, R. Launchbury, H. Hua, H.B. Callaghan, J.S. Carroll, R.C. Coombes, W. Zwart, L. Buluwela and S. Ali (see also Biotechnology).
Keywords for this news article include: Biotechnology, Europe, Genetics, Oncology, Cambridge, Breast Cancer, United Kingdom, Women's Health, Cancer Gene Therapy
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