By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Gram-Positive Bacteria. According to news reporting originating in Guilin, People's Republic of China, by NewsRx journalists, research stated, "A highly sensitive electrochemiluminescence (ECL) immunosensor for Cry1Ac was fabricated. The primary antibody anti-Cry1Ac was immobilized onto core-shell structural Fe(3)O(4)@Au nanoparticles."
The news reporters obtained a quote from the research from the Guilin University of Technology, "The antigen and glucose-oxidase-labeled secondary antibody were then successively combined to form sandwich-type immunocomplexes through a specific interaction. The magnetic particles loaded with sandwich immune complexes were attracted to a magnet-controlled glass carbon electrode (GCE) by an external magnet applied on top of the GCE. ECL was generated by the reaction between luminol and hydrogen peroxide derived from the enzymatic reaction in the presence of glucose. The sensors exhibited high sensitivity and a wide linear range for Bacillus thuringiensis Cry1Ac detection from 0 to 6 ng/mL, as well as a detection limit of 0.25 pg/mL (S/N=3)."
According to the news reporters, the research concluded: "The sensor is one of the most sensitive sensors for Cry1Ac, which can be easily renewed and conveniently used."
For more information on this research see: Electrogenerated chemiluminescence immunosensor for Bacillus thuringiensis Cry1Ac based on Fe3O4@Au nanoparticles. Journal of Agricultural and Food Chemistry, 2013;61(7):1435-40. (American Chemical Society - www.acs.org; Journal of Agricultural and Food Chemistry - www.pubs.acs.org/journal/jafcau)
Our news correspondents report that additional information may be obtained by contacting J. Li, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, People's Taiwan. Additional authors for this research include Q. Xu, X. Wei and Z. Hao (see also Gram-Positive Bacteria).
Keywords for this news article include: Asia, Guilin, Bacillaceae, Nanoparticle, Nanotechnology, Gram Positive Rods, Emerging Technologies, Bacillus thuringiensis, Gram Positive Bacteria, Gram-Positive Bacteria, People's Republic of China, Endospore Forming Bacteria, Gram Positive Endospore Forming Rods.
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC