By a News Reporter-Staff News Editor at Tuberculosis Week -- Investigators publish new report on Mycobacterium Infections. According to news reporting originating in Inchon, South Korea, by NewsRx journalists, research stated, "Upconversion nanoparticles (UCNPs), which are excited at near-infrared wavelength (980 nm), emit high-energy photons. Since UCNPs display a high signal-to-noise ratio and no photobleaching, they are extremely useful for diagnostic application."
The news reporters obtained a quote from the research from Gil Medical Center, "In this study, we applied UCNPs for detecting the 156110 sequence of the Mycobacterium tuberculosis complex (MTBC) and evaluated the feasibility of the system for use in molecular diagnostics. Using biotinylated primers, 156110 DNA PCR was performed and the PCR amplicon was then mixed with streptavidin-conjugated UCNPs, followed by intercalation with SYTOX Orange dye. Fluorescence detection for the Forster resonance energy transfer (FRET) of the UCNPs (UCNP-FRET) was then performed. The estimated lowest detection by UCNP-FRET was 10(2) copies/mu L of 156110 DNA (157 bp). The kappa agreement of the UCNP-FRET assay with conventional PCR was 0.8464 (95% confidence interval, 0.7442-0.9486) and false-negative results were reduced."
According to the news reporters, the research concluded: "Our results demonstrated the successful implementation of the UCNP-FRET system in detecting the 156110 sequence of the MTBC and its potential application for molecular diagnostics."
For more information on this research see: Upconversion nanoparticle-based Forster resonance energy transfer for detecting the IS6110 sequence of Mycobacterium tuberculosis complex in sputum. Biosensors & Bioelectronics, 2014;53():112-116. Biosensors & Bioelectronics can be contacted at: Elsevier Advanced Technology, Oxford Fulfillment Centre The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
Our news correspondents report that additional information may be obtained by contacting S.H. Hwang, Gil Med Center, Gachon Med Res Inst, Inchon, South Korea. Additional authors for this research include S.G. Im, H. Sung, S.S. Hah, V.T. Cong, D.H. Lee, S.J. Son and H.B. Oh (see also Mycobacterium Infections).
Keywords for this news article include: Asia, Inchon, South Korea, Actinobacteria, Mycobacteriaceae, Gram-Positive Rods, Gram-Positive Bacteria, Mycobacterium Infections, Actinomycetales Infections, Gram-Positive Asporogenous Rods
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC