By a News Reporter-Staff News Editor at Biotech Week -- Research findings on Enzymes and Coenzymes are discussed in a new report. According to news reporting out of Chongqing, People's Republic of China, by NewsRx editors, research stated, "In this work, a sandwich-type electrochemiluminescence (ECL) aptasensor for ultrasensitive detection of thrombin (TB) was designed based on mimicking bi-enzyme cascade catalysis to in situ generate coreactant of dissolved oxygen (O-2) for signal amplification. We utilized hollow Au nanoparticles (HAuNPs) as carriers to immobilize glucose oxidase nanoparticles (GOxNPs) and Pt nanoparticles (PtNPs) by electrostatic adsorption."
Our news journalists obtained a quote from the research from Southwest University, "Then, the detection aptamer of thrombin (TBA 2) was immobilized on the PtNPs/GOxNPs/HAuNPs nanocomplexes. Finally, hemin was intercalated into the TBA 2 to obtain the hemin/G-quadruplex structure. The hemin/G-quadruplex was an interesting DNAzyme that commonly mimiced horseradish peroxidase (HRP). Herein, GOxNPs, hemin/G-quadruplex and PtNPs could form mimicking bi-enzyme cascade catalysis system to in situ generate dissolved O-2 as coreactant in peroxydisulfate solution when the testing buffer contained proper amounts of glucose. This method had successfully overcome the disadvantage of difficulty to label the dissolved O-2 and realized the ECL signal amplification. The experiment proved that the aptasensor had good linear relationship on low concentration of TB."
According to the news editors, the research concluded: "The linear range was 1 x 10(-6)-10 nM, with a detection limit of 0.3 fM."
For more information on this research see: Amplified electrochemiluminescent aptasensor using mimicking bi-enzyme nanocomplexes as signal enhancement. Analytica Chimica Acta, 2014;809():47-53. Analytica Chimica Acta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Analytica Chimica Acta - www.elsevier.com/wps/product/cws_home/502681)
Our news journalists report that additional information may be obtained by contacting Y. Zhuo, Southwest University, Coll Chem & Chem Engn, Minist Educ, Key Lab Luminescence & Real Time Anal, Chongqing 400715, People's Republic of China. Additional authors for this research include M.N. Ma, Y.Q. Chai, M. Zhao and R. Yuan (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Heme, Drugs, Hemin, Therapy, Chongqing, Porphyrins, Nanoparticle, Nanotechnology, Biological Factors, Emerging Technologies, Enzymes and Coenzymes, People's Republic of China
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