News Column

Reports from University of Florida Describe Recent Advances in Peripheral Nerves

February 11, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Peripheral Nervous System are presented in a new report. According to news reporting out of Gainesville, Florida, by NewsRx editors, research stated, "While a wealth of literature for tissue-specific liposomes is emerging, optimal formulations to target the cells of the peripheral nervous system (PNS) are lacking. In this study, we asked whether a novel formulation of phospholipid-based liposomes could be optimized for preferential uptake by microvascular endothelia, peripheral neurons and Schwann cells."

Our news journalists obtained a quote from the research from the University of Florida, "Here, we report a unique formulation consisting of a phospholipid, a polymer surfactant and cholesterol that result in enhanced uptake by targeted cells. Using fluorescently labeled liposomes, we followed particle internalization and trafficking through a distinct route from dextran and escape from degradative compartments, such as lysosomes. In cultures of non-myelinating Schwann cells, liposomes associate with the lipid raft marker Cholera toxin, and their internalization is inhibited by disruption of lipid rafts or actin polymerization. In contrast, pharmacological inhibition of clathrin-mediated endocytosis does not significantly impact liposome entry. To evaluate the efficacy of liposome targeting in tissues, we utilized myelinating explant cultures of dorsal root ganglia and isolated diaphragm preparations, both of which contain peripheral neurons and myelinating Schwann cells. In these models, we detected preferential liposome uptake into neurons and glial cells in comparison to surrounding muscle tissue. Furthermore, in vivo liposome administration by intramuscular or intravenous injection confirmed that the particles were delivered to myelinated peripheral nerves. Within the CNS, we detected the liposomes in choroid epithelium, but not in myelinated white matter regions or in brain parenchyma."

According to the news editors, the research concluded: "The described nanoparticles represent a novel neurophilic delivery vehicle for targeting small therapeutic compounds, biological molecules, or imaging reagents into peripheral neurons and Schwann cells, and provide a major advancement toward developing effective therapies for peripheral neuropathies."

For more information on this research see: Liposomes to target peripheral neurons and Schwann cells. Plos One, 2013;8(11):e78724. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)

Our news journalists report that additional information may be obtained by contacting S. Lee, Dept. of Neuroscience, College of Medicine, University of Florida, Gainesville, Florida, United States. Additional authors for this research include A.T. Ashizawa, K.S. Kim, D.J. Falk and L. Notterpek (see also Peripheral Nervous System).

Keywords for this news article include: Florida, Neurons, Neuroglia, Gainesville, United States, Schwann Cells, Peripheral Nerves, North and Central America, Peripheral Nervous System.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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