By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Sulfur Amino Acids. According to news reporting originating in Guizhou, People's Republic of China, by NewsRx journalists, research stated, "In this work, a novel polyamidoamine (PAMAM) dendrimer-L-cysteine-hollow gold nanospheres nanocomposite was fabricated and used as the promoter for the peroxydisulfate/O-2 ECL system to detect the concentration of the tumor marker carcinoembryonic antigen (CEA). Herein, the carboxyl-terminated PAMAM dendrimers were decorated with L-cysteine (L-Cys) by EDC/NHS coupling chemistry."
The news reporters obtained a quote from the research from Bijie University, "Then, the hollow gold nanospheres (HGNPs) were employed as effective nano-carriers for the assembly of PAMAM-L-Cys via thiols-Au bonding, which was used for further loading of detection antibody (Ab(2)) to form the PAMAM-L-Cys-HGNPs-Ab(2) bioconjugates. In the presence of target CEA, the sandwiched immuno-structure can be formed between the capture anti-CEA antibodies (Ab(1)), which self-assembled on deposited gold modified electrode, and the Ab(2) on the PAMAM-L-Cys-HGNPs, thereby resulting in a proportional increase in ECL response, due to the significant enhancement of PAMAM-L-Cys-HGNPs toward peroxydisulfate/O-2 ECL system."
According to the news reporters, the research concluded: "As a result, a sandwich ECL assay for CEA detection was developed with excellent sensitivity of a large concentration variation from 20 fg/mL to 1.0 ng/mL and a detection limit of 6.7 fg mL(-1)."
For more information on this research see: Sandwich-format electrochemiluminescence assays for tumor marker based on PAMAM dendrimer-L-cysteine-hollow gold nanosphere nanocomposites. Biosensors & Bioelectronics, 2014;53():459-464. Biosensors & Bioelectronics can be contacted at: Elsevier Advanced Technology, Oxford Fulfillment Centre The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
Our news correspondents report that additional information may be obtained by contacting Y. Zhuo, Bijie Univ, Coll Chem & Chem Engn, Guizhou 551700, People's Republic of China. Additional authors for this research include G.F. Gui, Y.Q. Chai, N. Liao, K. Xiao and R. Yuan (see also Sulfur Amino Acids).
Keywords for this news article include: Asia, Guizhou, Cysteine, Nanocomposite, Nanotechnology, Sulfur Amino Acids, Neutral Amino Acids, Sulfhydryl Compounds, Emerging Technologies, People's Republic of China
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