By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- Researchers detail new data in Biotechnology. According to news reporting originating from Qingdao, People's Republic of China, by NewsRx correspondents, research stated, "A biosensing system is established for the multi-amplified detection of DNA or specific substrates of aptamers under isothermal conditions, which combines nicked rolling circle amplification (N-RCA) and beacon assisted amplification (BAA) with sensitive colorimetric technique by using DNAzymes as reporter units. According to the configuration, the analysis of DNA is accomplished by recognizing the target to capture nucleic acid-functionalized magnetic particles, followed by the self-assembly of the other two nucleic acids into multicomponent DNA supramolecular structure on magnetic particles."
Our news editors obtained a quote from the research from the Qingdao University of Science and Technology, "After magnetic separation, the circularization with ligase and the fragmentation with polymerase activate N-RCA and BAA in the presence of polymerase, dNTPs, and the nicking endonuclease, successively producing horseradish peroxidase (HRP)-mimicking DNAzymes that act as colorimetric reporter to catalyze the oxidation of ABTS(2-) by H2O2 in the presence of hemin. Under the optimized conditions, we obtain a wide dynamic range for DNA analysis over 6 orders of magnitude from 1.0 x 10(-14) to 1.0 x 10(-9) M with a low limit of detection of 6.8 x 10(-15) M. In the absence of a target, neither self-assembly of nucleic acids nor amplification process can be initiated, indicating an excellent selectivity of the proposed strategy."
According to the news editors, the research concluded: "Similarly, an analogous system is activated by cancer cells or lysozyme through cooperative self-assembly of nucleic acids on magnetic particles in the presence of respective substrates of aptamers to synthesize HRP-mimicking DNAzymes that give the readout signal for the recognition events, achieving LODs of 81 Ramos cells and 7.2 x 10(-15) M lysozyme, respectively."
For more information on this research see: Target-induced self-assembly of DNA nanomachine on magnetic particle for multi-amplified biosensing of nucleic acid, protein, and cancer cell. Biosensors & Bioelectronics, 2014;53():207-213. Biosensors & Bioelectronics can be contacted at: Elsevier Advanced Technology, Oxford Fulfillment Centre The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
The news editors report that additional information may be obtained by contacting S. Bi, Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Minist Educ, Key Lab Sensor Anal Tumor Marker, Qingdao 266042, People's Republic of China. Additional authors for this research include Y.Y. Cui, Y. Dong and N.B. Zhang (see also Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Qingdao, Oncology, DNA Research, Cancer Gene Therapy, People's Republic of China
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