By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on Enzymes and Coenzymes have been presented. According to news reporting originating from Changsha, People's Republic of China, by NewsRx correspondents, research stated, "Protein kinases are significant regulators in the cell signaling pathways, and it is still greatly desirable to achieve simple and quick kinase detection. Herein, we present a novel calorimetric gold nanoparticles (AuNPs)/peptide platform for probing the activity and inhibition of protein kinases based on phosphorylation-induced suppression of carboxypeptidase Y (CPY) cleavage."
Our news editors obtained a quote from the research from Hunan University, "This AuNPs/peptide platform can easily monitor the kinase activity by a UV-vis spectrometer or even by the naked eye. The feasibility of the method has been demonstrated by sensitive measurement of the cAMP-dependent protein kinase (PI(A) activity with a low detection limit of 0.232 mU/mu L and assessment of kinase inhibition by H-89 with an IC50 value of 18.13 nM. The assay was also successfully put into practice for the detection of kinase activity in cell lysate."
According to the news editors, the research concluded: "Because of its label-free, homogenous and calorimetric merits, the proposed assay presents great potential in high-throughput screening for kinase-targeted drug discovery."
For more information on this research see: A gold nanoparticles calorimetric assay for label-free detection of protein kinase activity based on phosphorylation protection against exopeptidase cleavage. Biosensors & Bioelectronics, 2014;53():295-300. Biosensors & Bioelectronics can be contacted at: Elsevier Advanced Technology, Oxford Fulfillment Centre The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
The news editors report that additional information may be obtained by contacting J. Zhou, Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, People's Republic of China. Additional authors for this research include X. Xu, X. Liu, H. Li, Z. Nie, M. Qing, Y. Huang and S. Yao (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Changsha, Nanotechnology, Protein Kinases, Gold Nanoparticles, Emerging Technologies, Enzymes and Coenzymes, People's Republic of China, Phosphotransferases (Alcohol Group Acceptor)
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