By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Germ Cells. According to news reporting from Olsztyn, Poland, by NewsRx journalists, research stated, "The antioxidant system in semen is composed of enzymes, low-molecular weight antioxidants and seminal plasma proteins. Loss of enzymatic activity of superoxide dismutase (SOD) during semen preservation may cause insufficient antioxidant defense of boar spermatozoa."
The news correspondents obtained a quote from the research from the University of Warmia and Mazury, "The aim of this study was to isolate and characterize SOD molecular forms from spermatozoa and to describe changes in SOD activity in boar sperm during preservation at 16°C. Sperm extracts were prepared from fresh or diluted semen and used for SOD purification or activity measurement. Ion-exchange chromatography and gel filtration was used to purify SOD molecular forms. BTS, Dilu Cell, M III and Vitasem were used as diluents for 5-day storage of semen at +16°C. The molecular form of SOD released from spermatozoa after cold shock and homogenization had a molecular weight of approximately 67kDa. The activity of the SOD form was the highest at pH 10 within the temperature range between 20 and 45°C. The enzymatic activity of form released after cold shock was inhibited by H2O2 and diethyldithiocarbamate (DDC; by 65 and 40%, respectively). The SOD form released by homogenization was inhibited by H2O2 and DDC (40%). The molecular form released after urea treatment was a 30kDa protein with maximum activity at 20°C and pH 10. Enzymatic activity of this form was inhibited by H2O2 by 35%, DDC by 80% and 2-mercaptoethanol by 15%. The antigenic determinants of SOD isolated from boar seminal plasma and spermatozoa were similar to each other."
According to the news reporters, the research concluded: "Susceptibility of spermatozoa to cold shock increased during storage, but the differences between extenders were statistically non-significant."
For more information on this research see: Superoxide dismutase (SOD) in boar spermatozoa: purification, biochemical properties and changes in activity during semen storage (16°C) in different extenders. Reproductive Biology, 2013;13(1):34-40. (BioMed Central - www.biomedcentral.com/; Reproductive Biology - www.rbej.com)
Our news journalists report that additional information may be obtained by contacting A. Orzo?ek, Dept. of Animal Biochemistry and Biotechnology, University of Warmia and Mazury, Olsztyn, Poland. Additional authors for this research include P. Wysocki, J. Strze?ek and W. Kordan (see also Germ Cells).
Keywords for this news article include: Poland, Europe, Anions, Olsztyn, Germ Cells, Biochemical, Spermatozoa, Biochemistry, Electrolytes, Oxidoreductases, Superoxide Dismutase, Enzymes and Coenzymes, Reactive Oxygen Species.
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC