By a News Reporter-Staff News Editor at Biotech Week -- A new study on Enzymes and Coenzymes is now available. According to news reporting from Guilin, People's Republic of China, by NewsRx journalists, research stated, "The profiling of microRNAs (miRNAs) is greatly significant for cellular events or disease diagnosis. Electrochemical methods for miRNAs analysis mostly can only measure one kind of miRNA, which is unambiguous to indicate the disease type and state."
The news correspondents obtained a quote from the research from the Guilin University of Technology, "Here a label-free and PCR-free electrochemical method is presented for multiplexed evaluation of miRNAs in a single-tube experiment. The method is based on the combination of the high base-mismatch selectivity of ligase chain reaction (LCR) and the remarkable voltammetric signature of electrochemical QDs barcodes. Two reporting probes of RP1 and RP2 were labeled with PbS and CdS quantum dots (QDs) to prepare PbS-RP1 and CdS-RP2 conjugates, and two capture probes of CP1 and CP2 were co-immobilized on magnetic beads (MBs) to fabricate MB-CP1CP2 conjugate. The miRNAs samples were simply incubated with MB-CP1CP2, PbS-RP1, and CdS-RP2 conjugates, and then added with T4 DNA ligase. After release of the disjoined QDs barcodes from the MB-conjugates, two target miRNAs of miR-155 and miR-27b were simultaneously detected by square wave voltammetry with linear ranges of 50 fM-30 pM and 50 fM-1050 pM, and limits of detection (LODs) of 12 fM and 31 fM (S/N=3). The method fulfilled the assay in less than 70 min, and showed acceptable testing recoveries for the determination of miRNAs in biological matrix. Currently there are rare reports about electrochemical multiplexed quantification of miRNAs."
According to the news reporters, the research concluded: "The method is likely to provide a new platform for identification of multiple miRNAs in a simple way."
For more information on this research see: A label-free and PCR-free electrochemical assay for multiplexed microRNA profiles by ligase chain reaction coupling with quantum dots barcodes. Biosensors & Bioelectronics, 2014;53():414-419. Biosensors & Bioelectronics can be contacted at: Elsevier Advanced Technology, Oxford Fulfillment Centre The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
Our news journalists report that additional information may be obtained by contacting W.Y. Zhu, Guilin Univ Technol, Coll Chem & Bioengn, Guilin 541004, People's Republic of China. Additional authors for this research include X.P. Su, X.Y. Gao, Z. Dai and X.Y. Zou (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Guilin, Ligases, Chemistry, Quantum Dots, Nanotechnology, Electrochemical, Quantum Physics, Emerging Technologies, Enzymes and Coenzymes, People's Republic of China
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