By a News Reporter-Staff News Editor at Biotech Business Week -- New research on Nanoparticles is the subject of a report. According to news reporting from Xi'an, People's Republic of China, by NewsRx journalists, research stated, "A highly sensitive and label-free electrochemical aptasensor was developed by employing click chemistry-assisted self-assembly of DNA aptamers on gold nanoparticles (GNPs)-modified screen-printed carbon electrodes (SPCEs). Lysozyme, a ubiquitous protein in mammals that is known as the 'body's own antibiotic', was chosen as the target protein and hexaammineruthenium(III) ([Ru(NH3)(6)](3+)) was used as the electrochemical indicator."
The news correspondents obtained a quote from the research from Shaanxi Normal University, "GNPs were first electrodeposited onto SPCEs and used as a platform for the immobilization of the aptamers. The aptasensor was fabricated by the self-assembly of 10-azidoundecan-1-thiol on GNPs-modified SPCE surfaces, followed by covalent attachment of anti-lysozyme binding aptamer (LBA) modified with alkynyl groups via click chemistry. The measurement was based on the displacement of [Ru(NH3)(6)](3+) from the LBA on the electrode in the presence of lysozyme and the decrease of square wave voltammetric response of surface-bound [Ru(NH3)(6)](3+). The decrease in the peak current was linear with the logarithm of the concentration of lysozyme in the range from 1.0 pg mL(-1) to 50.0 pg mL(-1) with a detection limit of 0.3 pg mL(-1). The apta sensor was successfully applied to the analysis of lysozyme in egg white samples."
According to the news reporters, the research concluded: "This work provides a promising immobilization strategy for the fabrication of biosensors and offers an alternative and sensitive method for protein detection."
For more information on this research see: Click chemistry-assisted self-assembly of DNA aptamer on gold nanoparticles-modified screen-printed carbon electrodes for label-free electrochemical aptasensor. Sensors and Actuators B-Chemical, 2014;192():558-564. Sensors and Actuators B-Chemical can be contacted at: Elsevier Science Sa, PO Box 564, 1001 Lausanne, Switzerland (see also Nanoparticles).
Our news journalists report that additional information may be obtained by contacting D.P. Xie, Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Appl Surface & Colloid Chem, Minist Educ, Xian 710062, People's Republic of China. Additional authors for this research include C.C. Li, S.G. Li, H.L. Qi, D. Xue, Q. Gao and C.X. Zhang.
Keywords for this news article include: Asia, Xi'an, Lysozyme, Chemistry, DNA Research, Nanotechnology, Electrochemical, Gold Nanoparticles, Emerging Technologies, People's Republic of China
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