By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Anions are presented in a new report. According to news originating from Budapest, Hungary, by NewsRx correspondents, research stated, "Enzymatic synthesis and hydrolysis of nucleoside phosphate compounds play a key role in various biological pathways, like signal transduction, DNA synthesis and metabolism. Although these processes have been studied extensively, numerous key issues regarding the chemical pathway and atomic movements remain open for many enzymatic reactions."
Our news journalists obtained a quote from the research from the Budapest University of Technology and Economics, "Here, using the Mason-Pfizer monkey retrovirus dUTPase, we study the dUTPase-catalyzed hydrolysis of dUTP, an incorrect DNA building block, to elaborate the mechanistic details at high resolution. Combining mass spectrometry analysis of the dUTPase-catalyzed reaction carried out in (H2O)-O-18 and quantum mechanics/molecular mechanics (QM/MM) simulation, we show that the nucleophilic attack occurs at the a-phosphate site. Phosphorus-31 NMR spectroscopy (P-31-NMR) analysis confirms the site of attack and shows the capability of dUTPase to cleave the dUTP analogue alpha,beta-imido-dUTP, containing the imido linkage usually regarded to be non-hydrolyzable. We present numerous X-ray crystal structures of distinct dUTPase and nucleoside phosphate complexes, which report on the progress of the chemical reaction along the reaction coordinate."
According to the news editors, the research concluded: "The presently used combination of diverse structural methods reveals details of the nucleophilic attack and identifies a novel enzyme-product complex structure."
For more information on this research see: Catalytic mechanism of alpha-phosphate attack in dUTPase is revealed by X-ray crystallographic snapshots of distinct intermediates, P-31-NMR spectroscopy and reaction path modelling. Nucleic Acids Research, 2013;41(22):10542-10555. Nucleic Acids Research can be contacted at: Oxford Univ Press, Great Clarendon St, Oxford OX2 6DP, England. (Oxford University Press - www.oup.com/; Nucleic Acids Research - nar.oxfordjournals.org)
The news correspondents report that additional information may be obtained from O. Barabas, Budapest Univ Technol & Econ, Dept. of Appl Biotechnol & Food Sci, Budapest, Hungary. Additional authors for this research include V. Nemeth, A. Bodor, A. Perczel, E. Rosta, Z. Kele, I. Zagyva, Z. Szabadka, V.I. Grolmusz, M. Wilmanns and B.G. Vertessy (see also Anions).
Keywords for this news article include: Europe, Anions, Hungary, Budapest, Phosphates, Phosphoric Acids
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