By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Amino Acids are discussed in a new report. According to news originating from Vienna, Austria, by NewsRx correspondents, research stated, "A novel analytical method using hydrophilic interaction liquid chromatography combined with electrospray tandem mass spectrometry for metabolic profiling of free, underivatized amino acids is presented. The separation uses a zwitterionic modified silica-based stationary phase with 1.8-mu m particle size functionalized with ammonium sulfonic acid groups."
Our news journalists obtained a quote from the research from the University of Natural Resources and Applied Life Science, "Quantification is based on external standard calibration using a Pichia pastoris cell extract grown on uniformly C-13 labeled glucose as an internal standard. The absolute limits of detection in the cellular matrix were in the subpicomolar range. Measurement accuracy was assessed by analyzing NIST Standard Reference Material 2389a, which provides certified values for 17 amino acids. The recovery of the amino acids ranged between 65 % (proline) and 120 % (lysine), with excellent repeatability precision below 2.5 % (n = 5). Only, cystine showed poor recovery (29 %) and repeatability precision (13 %). Generally, the long-term precision obtained by hydrophilic interaction liquid chromatography-tandem mass spectrometry was excellent, being on average less than 9 % over 20 h of measurement time. Moreover, the novel separation method had average repeatability and reproducibility of the chromatographic peak width over time periods of 20 h and 6 months of 8 and 15 %, respectively, demonstrating its high robustness in routine analysis of cellular samples."
According to the news editors, the research concluded: "Large concentration differences depending on the amino acid were found in the cell extracts, typically ranging from 0.002 nmol per milligram of cell dry weight (cystine) to 56 nmol per milligram of cell dry weight (arginine and glutamic acid)."
For more information on this research see: Metabolic profiling of amino acids in cellular samples via zwitterionic sub-2 mu m particle size HILIC-MS/MS and a uniformly C-13 labeled internal standard. Analytical and Bioanalytical Chemistry, 2014;406(3):915-922. Analytical and Bioanalytical Chemistry can be contacted at: Springer Heidelberg, Tiergartenstrasse 17, D-69121 Heidelberg, Germany. (Springer - www.springer.com; Analytical and Bioanalytical Chemistry - www.springerlink.com/content/1618-2642/)
The news correspondents report that additional information may be obtained from R. Guerrasio, Univ Nat Resources & Life Sci, Dept. of Biotechnol, A-1190 Vienna, Austria. Additional authors for this research include C. Haberhauer-Troyer, D. Mattanovich, G. Koellensperger and S. Hann (see also Amino Acids).
Keywords for this news article include: Vienna, Europe, Austria, Peptides, Proteins, Amino Acids
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