By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Serine have been published. According to news reporting originating in St. Petersburg, Russia, by NewsRx journalists, research stated, "A reliable and high throughput high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed and validated for determining levels of the antitubercular drug-d -cycloserine in human plasma. Plasma samples analyte with an internal standard (IS) (niacin) were prepared by solid-phase extraction using Waters Oasis MCX cartridges."
The news reporters obtained a quote from the research from St. Petersburg State Polytechnic University, "The chromatographic separation was performed using the HILIC mode on a YMC-Pack SIL-06 column (150 x 4.6 mm; 3 mu m) under isocratic conditions. The run time of analysis was 5 min. The mobile phase consisted of methanol, propanol-2 and 0.075 % trifluoroacetic acid (66.5:28.5:5, v/v/v). Protonated ions formed by turbo ion spray in positive mode were used to detect the analyte and the IS. MS/MS detection was used to monitor the fragmentation of 103-75 m/z for cycloserine and 124 to 80 m/z for niacin (IS) on an API 4000 (AB Sciex) triple quadrupole mass spectrometer. A linear dynamic range of 0.3-30 mu g/mL was established for cycloserine using 0.2 mL human plasma and a 1 mu L injection volume. The mean relative recovery of cycloserine and niacin were 77.2 and 82.4 %, respectively. The procedure of sample preparation was consistent and reproducible (precision, 0.8-3.4 %; accuracy, 93.8-104.9 %)."
According to the news reporters, the research concluded: "The method was validated in accordance with requirements of the European Medicines Agency and successfully applied to a bioequivalence study of 250 mg tablet formulations in 23 healthy human subjects."
For more information on this research see: Development and validation of a LC-MS/MS method for D-cycloserine determination in human plasma for bioequivalence study. Analytical and Bioanalytical Chemistry, 2014;406(3):923-927. Analytical and Bioanalytical Chemistry can be contacted at: Springer Heidelberg, Tiergartenstrasse 17, D-69121 Heidelberg, Germany. (Springer - www.springer.com; Analytical and Bioanalytical Chemistry - www.springerlink.com/content/1618-2642/)
Our news correspondents report that additional information may be obtained by contacting D.V. Yaroshenko, St Petersburg State Polytechnic Univ, Bioanalyt Center CSU Analyt Spectrometry, St Petersburg 198220, Russia. Additional authors for this research include A.V. Grigoriev and A.A. Sidorova (see also Serine).
Keywords for this news article include: Russia, Eurasia, Cycloserine, St. Petersburg
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