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Studies from University of Guilan Have Provided New Data on Biological Toxins

March 4, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Biological Factors have been published. According to news reporting out of Rasht, Iran, by NewsRx editors, research stated, "Enterotoxigenic Escherichia con (ETEC) are the most common cause of diarrhea among children. Colonization factors and enterotoxins are the major ETEC candidate vaccines."

Our news journalists obtained a quote from the research from the University of Guilan, "Since protection against ETEC mostly occurs by induction of IgA antibodies, much effort is focused on the development of oral vaccines. In this study oral immunogenicity of a poly(lactic-co-glycolic acid) (PLGA) encapsulated chimeric protein containing CfaB, CstH, CotA and LTB (Heat-labile B subunit) was investigated. The protein was encapsulated in PLGA by double emulsion method and nanoparticles were characterized physicochemically. Immunogenicity was assessed by evaluating IgG1, IgG2 and IgA titers after BALB/c mice vaccination. Non aggregated nanoparticles had a spherical shape with an average particle size of 252.7 +/- 23 nm and 91.96 +/- 4.4% of encapsulation efficiency. Western blotting showed maintenance of the molecular weight and antigenicity of the released protein. Oral immunization of mice induced serum IgG and fecal IgA antibody responses. Immunization induced protection against ETEC binding to Caco-2 cells. The effect of LT toxin on fluid accumulation in ileal loops was neutralized by inhibition of enterotoxin binding to GM1-ganglosides. Delivery of the chimeric protein in PLGA elicited both systemic and mucosal immune responses."

According to the news editors, the research concluded: "The findings could be exploited to development of oral multi-component ETEC prophylactic measures."

For more information on this research see: A PLGA-encapsulated chimeric protein protects against adherence and toxicity of enterotoxigenic Escherichia coil. Microbiological Research, 2014;169(2-3):205-212. Microbiological Research can be contacted at: Elsevier Gmbh, Urban & Fischer Verlag, Office Jena, P O Box 100537, 07705 Jena, Germany. (Elsevier - www.elsevier.com; Microbiological Research - www.elsevier.com/wps/product/cws_home/701785)

Our news journalists report that additional information may be obtained by contacting S. Nazarian, Univ Guilan, Fac Sci, Dept. of Biol, Rasht, Iran. Additional authors for this research include S.L.M. Gargari, I. Rasooli, S. Hasannia and N. Pirooznia (see also Biological Factors).

Keywords for this news article include: Iran, Asia, Rasht, Nanoparticle, Nanotechnology, Biological Factors, Emerging Technologies

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Source: Life Science Weekly


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