By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Biotechnology is now available. According to news reporting originating from Inchon, South Korea, by NewsRx correspondents, research stated, "We previously completed whole-genome sequencing of a rare actinomycete named Sebekia benihana, and identified the complete S. benihana cytochrome P450 complement (CYPome), including 21 cytochrome P450 hydroxylase (CYP), seven ferredoxin (FD), and four ferredoxin reductase (FDR) genes. Through targeted CYPome disruption, a total of 32 S. benihana CYPome mutants were obtained."
Our news editors obtained a quote from the research from Inha University, "Subsequently, a novel cyclosporine A region-specific hydroxylase was successfully determined to be encoded by a CYP-sb21 gene by screening the S. benihana CYPome mutants. Here, we report that S. benihana is also able to mediate vitamin D-3 (VD3) hydroxylation. Among the 32 S. benihana CYPome mutants tested, only a single S. benihana CYP mutant, Delta CYP-sb3a, failed to show regio-specific hydroxylation of VD3 to 25-hydroxyvitamin D-3 and 1 alpha,25-dihydroxyvitamin D-3. Moreover, the VD3 hydroxylation activity in the Delta CYP-sb3a mutant was restored by CYP-sb3a gene complementation. Since all S. benihana FD and FDR disruption mutants maintained VD3 hydroxylation activity, we conclude that CYP-sb3a, a member of the bacterial CYP107 family, is the only essential component of the in vivo regio-specific VD3 hydroxylation process in S. benihana."
According to the news editors, the research concluded: "Expression of the CYP-sb3a gene exhibited VD3 hydroxylation in the VD3 non-hydroxylating Streptomyces coelicolor, implying that the regio-specific hydroxylation of VD3 is carried out by a specific P450 hydroxylase in S. benihana."
For more information on this research see: Identification of a vitamin D-3-specific hydroxylase genes through actinomycetes genome mining. Journal of Industrial Microbiology & Biotechnology, 2014;41(2):265-273. Journal of Industrial Microbiology & Biotechnology can be contacted at: Springer Heidelberg, Tiergartenstrasse 17, D-69121 Heidelberg, Germany. (Springer - www.springer.com; Journal of Industrial Microbiology & Biotechnology - www.springerlink.com/content/1367-5435/)
The news editors report that additional information may be obtained by contacting J.G. Ban, Inha University, Dept. of Biol Engn, Inchon 402751, South Korea. Additional authors for this research include H.B. Kim, M.J. Lee, P. Anbu and E.S. Kim (see also Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Inchon, South Korea, Hydroxylase, Enzymes and Coenzymes
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