By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Proteins. According to news reporting originating in Cluj Napoca, Romania, by NewsRx journalists, research stated, "Bioactive glasses belonging to the 56SiO2·(40 -x)CaO·4P2O5·xAg2O system, with x=0, 2, and 8 mol %, were surface functionalized with the protein coupling agent glutaraldehyde (GA) and further evaluated in terms of hemoglobin affinity. The bare and GA-functionalized samples were investigated before and after protein attachment, by electron paramagnetic resonance (EPR) spectroscopy combined with spin-labeling procedure."
The news reporters obtained a quote from the research from Babes-Bolyai University, "Methanethiosulfonate spin label was used to explore the local environment of ?-93 cysteine in horse hemoglobin, in terms of spin label side chain mobility. The EPR simulation methods were employed to quantify the rotational correlational times and fraction of the immobilized spin labels. The EPR absorption spectrum was further exploited to estimate the amount of hemoglobin loaded on the substrates. The surface elemental composition obtained by X-ray photoelectron spectroscopy revealed similar tendency in terms of surface coverage. Changes in surface architecture, that is, changes in surface morphology after protein coverage, were observed by scanning electron microscopy."
According to the news reporters, the research concluded: "It was concluded that GA improves the stability of protein attachment and induces polymerization of hemoglobin molecules."
For more information on this research see: The attachment affinity of hemoglobin toward silver-containing bioactive glass functionalized with glutaraldehyde. The Journal of Physical Chemistry B, 2013;117(51):16558-64 (see also Proteins).
Our news correspondents report that additional information may be obtained by contacting C. Gruian, Faculty of Physics & Institute of Interdisciplinary Research in Bio-Nano-Sciences, Babes-Bolyai University , Cluj-Napoca 400084, Romania. Additional authors for this research include A. Vulpoi, E. Vanea, B. Oprea, H.J. Steinhoff and S. Simon.
Keywords for this news article include: Europe, Romania, Cluj Napoca, Hemoglobins, Blood Proteins.
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