By a News Reporter-Staff News Editor at Cancer Weekly -- Investigators publish new report on Nanoparticles. According to news reporting from Chengdu, People's Republic of China, by NewsRx journalists, research stated, "Superparamagnetic magnetite nanoparticles (MNPs) of different surface properties are incubated in complicated living fluid, including fetal bovine serum solution, cell complete culture medium and cell culture system with/without serum, to investigate the alteration of protein corona and its impact on cell internalization. The MNPs prepared by co-precipitation method are functionalized with L-Lysine (Lys), Glucosamic acid (GA) to obtain amine, carboxyl and hydroxyl groups, separately."
The news correspondents obtained a quote from the research from Sichuan University, "All the particles adsorb serum proteins to form MNPs-protein complexes with the surface charge changing into negative. 1D SDS/PAGE gel images analysis indicates that the composition and content of hard protein corona on the surface of NPs are related to their functional groups and agglomeration, and the total amount of protein in the medium. In cell culture system, particles not only adsorb serum proteins, but also associate with cytosolic proteins arising from HepG2 and L02 cells. GA modified MNPs (MNPs-GA) exhibit bovine serum albumin anti-adsorption capability because of the terminal hydroxyl and carboxyl groups. MNPs-GA also shows the highest cellular uptake and label efficiency compared with uncoated MNPs and Lys modified MNPs, due to larger aggregates formation and specific protein corona composition, rather than commonly approved electrostatic interaction between particles and cells."
According to the news reporters, the research concluded: "For the first time, our results provide visualized reports on previously neglected, but indispensable protein corona of the MNPs after interaction with both healthy and cancer cells, suggesting that cytosolic protein corona from cells and aggregation of particles are important factors needed to be account for on studying the nano-bio interface."
For more information on this research see: Protein corona on magnetite nanoparticles and internalization of nanoparticle-protein complexes into healthy and cancer cells. Archives of Pharmacal Research, 2014;37(1):129-41. Archives of Pharmacal Research can be contacted at: Springer, 233 Spring Street, New York, NY 10013, USA. (Springer - www.springer.com; Archives of Pharmacal Research - www.springerlink.com/content/0253-6269/)
Our news journalists report that additional information may be obtained by contacting W. Jiang, National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, Sichuan, People's Taiwan. Additional authors for this research include K. Lai, Y. Wu and Z. Gu (see also Nanoparticles).
Publisher contact information for the journal Archives of Pharmacal Research is: Springer, 233 Spring Street, New York, NY 10013, USA.
Keywords for this news article include: Asia, Cancer, Chengdu, Oncology, Peptides, Proteins, Amino Acids, Nanotechnology, Emerging Technologies, People's Republic of China.
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC