By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Enzymes and Coenzymes. According to news reporting originating from Taejon, South Korea, by NewsRx correspondents, research stated, "Brine shrimp (Artemia saline) belong to a group of crustaceans that feed on microalgae and require a cellulase enzyme that can be used in ethanol production from marine algae. Protein with potential cellulase activity was purified and the activity analyzed under different conditions."
Our news editors obtained a quote from the research from the Korea Research Institute of Bioscience and Biotechnology, "After initial identification of cellulase activity by CMC cellulase, surface sterilization and PCR using 16s rRNA primers was conducted to confirm that the cellulase activity was not produced from contaminating bacteria. The enzyme was purified by ammonium sulfate fractionation, gel filtration, and ion exchange chromatography. After the final purification, a 70-fold increase in specific enzyme activity was observed. SDS-PAGE results revealed that the cellulase enzyme had a molecular mass of 96 kDa. Temperature, pH, and salinity values were found to be optimal at 55 degrees C, pH 8.0, and 600 mM NaCl, respectively. Specifically, the enzyme showed a fivefold increase in enzyme activity in seawater compared to 600 mM NaCl in phosphate buffer."
According to the news editors, the research concluded: "Further analysis of the purified enzyme by molecular spectrometry showed no match to known cellulases, indicating this enzyme could be a novel halophilic cellulase that can be used for the production of bioethanol from marine macroalgae."
For more information on this research see: Purification and characterization of a carboxymethyl cellulase from Artemia salina. Biochemical and Biophysical Research Communications, 2014;443(1):194-199. Biochemical and Biophysical Research Communications can be contacted at: Academic Press Inc Elsevier Science, 525 B St, Ste 1900, San Diego, CA 92101-4495, USA. (Elsevier - www.elsevier.com; Biochemical and Biophysical Research Communications - www.elsevier.com/wps/product/cws_home/622790)
The news editors report that additional information may be obtained by contacting H.W. Zin, Korea Res Inst Biosci & Biotechnol, Taejon 305806, South Korea. Additional authors for this research include K.H. Park and T.J. Choi (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Taejon, Cellulases, South Korea, Glucosidases, Glycoside Hydrolases, Enzymes and Coenzymes
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC