By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Gram-Positive Bacteria is the subject of a report. According to news reporting from Karaj, Iran, by NewsRx journalists, research stated, "Streptomyces strain C-2012 is a salt tolerant biocontrol PGPR that has been isolated from Iranian soil. The main aim of current study was finding strain C-2012 taxonomic position and to find the genes which are potentially involved in salt tolerance phenotype."
The news correspondents obtained a quote from the research from Biotechnology Research Institute, "Strain C-2012 chemotaxonomic, morphological and molecular characteristics indicate that this strain is a member of the genus Streptomyces. Phylogenetic analyses based on an almost complete 16S rRNA gene sequence revealed that this strain is closely related to Streptomyces rimosusJCM 4667T' Also, DNA-DNA hybridization test estimated 74% relatedness between two strains and confirmed that C-2012 is a strain of S. rimosus. In order to find novel genes that are differentially expressed in response to the salt treatment, cDNA-AFLP was carried out. One of the selected expressed sequence tags (TDF-1) was found to be homologous to Ion gene which produces a bacterial ATP-dependent proteases (proteases LA). Lon gene expression was induced following 450 mM salt (NaCl) treatment and its expression level was further (5.2-fold) increased in response to salt when ectoine was added to the medium. These results suggest that two protein protection systems including ectoine and ATP-dependent proteases synergistically are related."
According to the news reporters, the research concluded: "NaCl stress also caused an enhancement in the activity of extracellular protease."
For more information on this research see: Taxonomic study of a salt tolerant Streptomyces sp strain C-2012 and the effect of salt and ectoine on lon expression level. Microbiological Research, 2014;169(2-3):232-238. Microbiological Research can be contacted at: Elsevier Gmbh, Urban & Fischer Verlag, Office Jena, P O Box 100537, 07705 Jena, Germany. (Elsevier - www.elsevier.com; Microbiological Research - www.elsevier.com/wps/product/cws_home/701785)
Our news journalists report that additional information may be obtained by contacting A. Sadeghi, Agr Biotechnol Res Inst Iran, Microbial Biotechnol & Biosafety Department, Karaj, Iran. Additional authors for this research include B.M. Soltani, G.S. Jouzani, E. Karimi, M.K. Nekouei and M. Sadeghizadeh (see also Gram-Positive Bacteria).
Keywords for this news article include: Iran, Asia, Karaj, Protease, Treatment, Actinomycetales, Streptomycetaceae, Enzymes and Coenzymes, Gram-Positive Bacteria, Gram-Positive Endospore-Forming Rods
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