News Column

Findings from Monterey Bay Aquarium Research Institute Broadens Understanding of Biochemical and Biophysical Research

March 4, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Life Science Research have been published. According to news reporting from Moss Landing, California, by NewsRx journalists, research stated, "Calcium-binding photoproteins have been discovered in a variety of luminous marine organisms [1]. Recent interest in photoproteins from the phylum Ctenophora has stemmed from cloning and expression of several photoproteins from this group [2-5]."

The news correspondents obtained a quote from the research from Monterey Bay Aquarium Research Institute, "Additional characterization has revealed unique biochemical properties found only in ctenophore photoproteins, such as inactivation by light. Here we report the cloning, expression, and characterization of the photoprotein responsible for luminescence in the deep-sea ctenophore Bathocyroe fosteri. This animal was of particular interest due to the unique broad color spectrum observed in live specimens [6]. Full-length sequences were identified by BLAST searches of known photoprotein sequences against Bathocyroe transcripts obtained from 454 sequencing. Recombinantly expressed Bathocyroe photoprotein (BfosPP) displayed an optimal coelenterazine-loading pH of 8.5, and produced calcium-triggered luminescence with peak wavelengths closely matching the 493 nm peak observed in the spectrum of live B. fosteri specimens. Luminescence from recombinant BfosPP was inactivated most efficiently by UV and blue light. Primary structure alignment of BfosPP with other characterized photoproteins showed very strong sequence similarity to other ctenophore photoproteins and conservation of EF-hand motifs."

According to the news reporters, the research concluded: "Both alignment and structural prediction data provide more insight into the formation of the coelenterazine-binding domain and the probable mechanism of photoinactivation."

For more information on this research see: Expression and characterization of the calcium-activated photoprotein from the ctenophore Bathocyroe fosteri: insights into light-sensitive photoproteins. Biochemical and Biophysical Research Communications, 2013;431(2):360-6. (Elsevier - www.elsevier.com; Biochemical and Biophysical Research Communications - www.elsevier.com/wps/product/cws_home/622790)

Our news journalists report that additional information may be obtained by contacting M.L. Powers, Monterey Bay Aquarium Research Institute, 7700 Sandholdt Road, Moss Landing, CA 95039, United States. Additional authors for this research include A.G. McDermott, N.C. Shaner and S.H Haddock (see also Life Science Research).

Keywords for this news article include: California, Moss Landing, United States, Life Science Research, North and Central America.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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