By a News Reporter-Staff News Editor at Science Letter -- Research findings on Chalcogens are discussed in a new report. According to news reporting originating in St. Gallen, Switzerland, by NewsRx journalists, research stated, "The release of reactive oxygen species (ROS) during the electron transport of mitochondrial aerobic respiration is the major source of ROS. However, contact between cells and nanoparticles (NPs) can also induce release of ROS, leading to an imbalance towards the pro-oxidative state."
The news reporters obtained a quote from the research from Swiss Federal Laboratories for Materials Science and Technology (EMPA), "At low levels of ROS production, cells initiate a protective response to guarantee their survival, but an excess of ROS can damage cellular compounds such as membranes and various organelles, or directly cause genotoxicity. Thus an elevated level of ROS is an important indicator of cellular stress and an accurate recording of this parameter would be very informative. ROS can be measured by various assays, but all known assays measuring and quantifying ROS possess certain weaknesses. The problems and challenges of quantitatively detecting ROS in vitro using the 2,7-dichlorodihydrofluorescein (DCF) assay is discussed as an example. In addition, we debate the difficulties in finding a suitable and stable chemical reaction control for the DCF assay (or other ROS-detecting assays). As a conclusion, we believe that using 3-morpholinosydnonimine hydrochloride (Sin-1) as a ROS inducer in the DCF assay is feasible only qualitatively."
According to the news reporters, the research concluded: "However, a quantitative measurement of the absolute amount of ROS produced and a quantitative comparison between experiments is (at the moment) impossible."
For more information on this research see: Comparability of in Vitro Tests for Bioactive Nanoparticles: A Common Assay to Detect Reactive Oxygen Species as an Example. International Journal of Molecular Sciences, 2013;14(12):24320-24337. International Journal of Molecular Sciences can be contacted at: Mdpi Ag, Postfach, Ch-4005 Basel, Switzerland (see also Chalcogens).
Our news correspondents report that additional information may be obtained by contacting M. Roesslein, Empa, Swiss Fed Labs Mat Sci & Technol, Res Focus Area Hlth & Performance, CH-9014 St Gallen, Switzerland. Additional authors for this research include C. Hirsch, J.P. Kaiser, H.F. Krug and P. Wick.
Keywords for this news article include: Europe, St. Gallen, Chalcogens, Switzerland, Nanoparticle, Nanotechnology, Oxygen Compounds, Emerging Technologies, Reactive Oxygen Species
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