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Study Results from Shandong Academy of Sciences Broaden Understanding of Molecular Biology

February 25, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Life Science Research is now available. According to news reporting out of Jinan, People's Republic of China, by NewsRx editors, research stated, "PH-Zone refining counter-current chromatography was successfully applied to the preparative isolation and purification of six alkaloids from the ethanol extracts of Uncaria macrophylla Wall. Because of the low content of alkaloids (about 0.2%, w/w) in U. macrophylla Wall, the target compounds were enriched by pH-zone refining counter-current chromatography using a two-phase solvent system composed of petroleum ether-ethyl acetate-isopropanol-water (2: 6: 3:9, v/v), adding 10 mM triethylamine in organic stationary phase and 5 mM hydrochloric acid in aqueous mobile phase."

Our news journalists obtained a quote from the research from the Shandong Academy of Sciences, "Then pH-zone refining counter-current chromatography using the other two-phase solvent system was used for final purification. Six target compounds were finally isolated and purified by following two-phase solvent system composed of methyl tert-butyl ether (MTBE)-acetonitrile-water (4:0.5:5, v/v), adding triethylamine (TEA) (10 mM) to the organic phase and HCl (5 mM) to aqueous mobile phase."

According to the news editors, the research concluded: "The separation of 2.8 g enriched total alkaloids yielded 36 mg hirsutine, 48 mg hirsuteine, 82 mg uncarine C, 73 mg uncarine E, 163 mg rhynchophylline, and 149 mg corynoxeine, all with purities above 96% as verified by HPLC Their structures were identified by electrospray ionization-mass spectrometry (ESI-MS) and H-1-NMR spectroscopy."

For more information on this research see: Preparative Separation of Six Rhynchophylla Alkaloids from Uncaria macrophylla Wall by pH-Zone Refining Counter-Current Chromatography. Molecules, 2013;18(12):15490-15500. Molecules can be contacted at: Mdpi Ag, Postfach, Ch-4005 Basel, Switzerland. (Springer - www.springer.com; Molecules - www.springerlink.com/content/1420-3049/)

Our news journalists report that additional information may be obtained by contacting Q.H. Zhang, Shandong Academy Sci, Shandong Anal & Test Center, TCM Proc Control Res Center, Jinan 250014, People's Republic of China. Additional authors for this research include C.H. Lin, W.J. Duan, X. Wang and A.Q. Luo (see also Life Science Research).

Keywords for this news article include: Asia, Jinan, Life Science Research, People's Republic of China

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Source: Life Science Weekly


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