By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on 3-Hydroxybutyric Acid have been presented. According to news reporting out of Tokyo, Japan, by NewsRx editors, research stated, "Genes encoding 3-hydroxybutyrate oligomer hydrolase (PhaZc) and 3-hydroxybutyrate dehydrogenase (Hbd) were isolated from Paracoccus denitrificans. PhaZc and Hbd were overproduced as His-tagged proteins in Escherichia coli and purified by affinity and gel filtration chromatography."
Our news journalists obtained a quote from the research from the Tokyo University of Agriculture and Technology, "Purified His-tagged proteins had molecular masses of 31 kDa and 120 kDa (a tetramer of 29-kDa subunits). The His-tagged PhaZc hydrolyzed not only 3-hydroxybutyrate oligomers but also 3-hydroxyvalerate oligomers. The His-tagged Hbd catalyzed the dehydrogenation of 3-hydroxyvalerate as well as 3-hydroxybutyrate. When both enzymes were included in the same enzymatic reaction system with 3-hydroxyvalerate dimer, sequential reactions occurred, suggesting that PhaZc and Hbd play an important role in the intracellular degradation of poly(3-hydroxyvalerate). When the phaZc gene was disrupted in P. denitrificans by insertional inactivation, the mutant strain lost PhaZc activity. When the phaZc-disrupted P. denitrificans was complemented with phaZc, PhaZc activity was restored. These results suggest that P. denitrificans carries a single phaZc gene."
According to the news editors, the research concluded: "Disruption of the phaZc gene in P. denitrificans affected the degradation rate of PHA."
For more information on this research see: 3-Hydroxybutyrate Oligomer Hydrolase and 3-Hydroxybutyrate Dehydrogenase Participate in Intracellular Polyhydroxybutyrate and Polyhydroxyvalerate Degradation in Paracoccus denitrificans. Applied and Environmental Microbiology, 2014;80(3):986-993. Applied and Environmental Microbiology can be contacted at: Amer Soc Microbiology, 1752 N St NW, Washington, DC 20036-2904, USA. (American Society for Microbiology - www.asm.org; Applied and Environmental Microbiology - aem.asm.org)
Our news journalists report that additional information may be obtained by contacting J. Lu, Tokyo University of Agriculture & Technology, United Grad Sch Agr Sci, Dept. of Appl Life Sci, Tokyo, Japan. Additional authors for this research include A. Takahashi and S. Ueda (see also 3-Hydroxybutyric Acid).
Keywords for this news article include: Asia, Tokyo, Japan, Hydrolases, Paracoccus, Dehydrogenase, Carboxylic Acids, Hydroxybutyrates, Rhodobacteraceae, Organic Chemicals, Alphaproteobacteria, 3-Hydroxybutyric Acid, Enzymes and Coenzymes, Gram-Negative Bacteria, Gram-Negative Aerobic Bacteria, Gram-Negative Aerobic Rods and Cocci
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