By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on DNA Research are discussed in a new report. According to news reporting out of Changsha, People's Republic of China, by NewsRx editors, research stated, "A simple and sensitive gap-electrical biosensor based on self-catalytic growth of unmodified gold nanoparticles (AuNPs) as conductive bridges has been developed for amplifying DNA hybridization events. In this strategy, the signal amplification degree of such conductive bridges is closely related to the variation of the glucose oxidase (GOx)-like catalytic activity of AuNPs upon interaction with single- and double-stranded DNA (ssDNA and dsDNA), respectively."
Our news journalists obtained a quote from the research from Hunan University, "In the presence of target DNA, the obtained dsDNA product cannot adsorb onto the surface of AuNPs due to electrostatic interaction, which makes the unmodified AuNPs exhibit excellent GOx-like catalytic activity. Such catalytic activity can enlarge the diameters of AuNPs in the glucose and HAuCl4 solution and result in a connection between most of the AuNPs and a conductive gold film formation with a dramatically increased conductance. For the control sample, the catalytic activity sites of AuNPs are fully blocked by ssDNA due to the noncovalent interaction between nucleotide bases and AuNPs. Thus, the growth of the assembled AuNPs will not happen and the conductance between microelectrodes will be not changed. Under the optimal experimental conditions, the developed strategy exhibited a sensitive response to target DNA with a high signal-to-noise ratio. Moreover, this strategy was also demonstrated to provide excellent differentiation ability for single-nucleotide polymorphism."
According to the news editors, the research concluded: "Such performances indicated the great potential of this label-free electrical strategy for clinical diagnostics and genetic analysis under real biological sample separation."
For more information on this research see: Self-Catalytic Growth of Unmodified Gold Nanoparticles as Conductive Bridges Mediated Gap-Electrical Signal Transduction for DNA Hybridization Detection. Analytical Chemistry, 2014;86(2):1178-1185. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
Our news journalists report that additional information may be obtained by contacting J. Zhang, Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, People's Republic of China. Additional authors for this research include H.G. Nie, Z. Wu, Z. Yang, L.J. Zhang, X.J. Xu and S.M. Huang (see also DNA Research).
Keywords for this news article include: Asia, Changsha, DNA Research, Nanotechnology, Gold Nanoparticles, Emerging Technologies, People's Republic of China
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