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Studies from Central Salt and Marine Chemicals Research Institute Yield New Data on Escherichia Coli

February 25, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Proteobacteria is the subject of a report. According to news reporting from Bhavnagar, India, by NewsRx journalists, research stated, "Salicornia brachiata is an extreme halophyte considered to be a rich source of stress responsive genes and an EST database revealed that 30% of its genes are uncharacterized. In order to ascertain its function, a gene (Sal-E-56) of unknown function was made full length using RACE, cloned and characterized."

The news correspondents obtained a quote from the research from Central Salt and Marine Chemicals Research Institute, "The full length gene (873 bp; accession no. KF164282) contained an open reading frame (ORF) of 486 bp encoding for a protein that belongs to the universal stress protein (USP) family that was named SbUSP. The SbUSP interacted with adenosine monophosphate and exhibited characteristic motifs, phosphorylation, glycosylation and ATP binding sites. Further, in-silico analyses suggested a probable role in metabolic process of phosphate-containing compounds including signal transduction. In planta transcript profiling exhibited a significant expression response (7.8-fold) to salt stress, additionally abundant of SbUSP transcripts were observed during drought, heat and cold stress, reaching a maximum increase of 3.66-, 2.64- and 2.14-fold, respectively, at 12 or 24 h. The heterologous expression of this gene in Escherichia coli provided enhanced stress tolerance and recombinant cells have higher growth rate compared to vector alone and showed growth at up to a 10(-5) dilution in the spot assay. It was predicted that SbUSP may be directly involved in tolerance mechanisms or function as a molecular switch (signaling molecule) to activate the stress adaptive mechanisms."

According to the news reporters, the research concluded: "However, further investigation will be required to determine its role as a molecular switch and mode of action during stress."

For more information on this research see: Heterologous expression of an uncharacterized universal stress protein gene (SbUSP) from the extreme halophyte, Salicornia brachiata, which confers salt and osmotic tolerance to E. coli. Gene, 2014;536(1):163-170. Gene can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier -; Gene -

Our news journalists report that additional information may be obtained by contacting P. Udawat, CSIR, Cent Salt & Marine Chem Res Inst, Discipline Marine Biotechnol & Ecol, Bhavnagar 364002, Gujarat, India. Additional authors for this research include A. Mishra and B. Jha (see also Proteobacteria).

Keywords for this news article include: Asia, India, Bhavnagar, Escherichia, Nanotechnology, Enterobacteriaceae, Molecular Switches, Gammaproteobacteria, Emerging Technologies

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Source: Life Science Weekly

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