By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on DNA Research. According to news reporting out of Berlin, Germany, by NewsRx editors, research stated, "Nucleic acid-templated reactions are frequently explored tools in nucleic acid diagnosis. To enable a separation-free DNA detection, the reactive probe molecules require conjugation with reporter groups that provide measurable changes of an observable parameter upon reaction."
Our news journalists obtained a quote from the research from the University of Berlin, "A widely used, generic read-out method is based on fluorescence resonance energy transfer (FRET) between two appended dyes. Yet, spectral cross-talk usually limits the achievable enhancements of the FRET signal in DNA-directed chemistries. We describe a DNA-triggered transfer reaction which provides for strong increases of a fluorescent signal caused by FRET. The method may involve DNA- and PNA-based probes and is based upon a proximity. triggered transfer reaction which leads to the covalent fixation of a fluorescence dye on the surface of a quantum dot (QD). The transfer reaction brings the dye closer to the QD than hybridization alone. The resulting FRET signal is a specific monitor of the reaction and allows efficient discrimination of single base mismatched templates."
According to the news editors, the research concluded: "Of note, the 35-fold increase of the FRET signal is measured at 310 nm apparent Stokes shift and turnover in template provides a means for signal amplification."
For more information on this research see: DNA-Triggered Dye Transfer on a Quantum Dot. Bioconjugate Chemistry, 2014;25(1):18-23. Bioconjugate Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Bioconjugate Chemistry - www.pubs.acs.org/journal/bcches)
Our news journalists report that additional information may be obtained by contacting J. Michaelis, Univ Berlin, Inst Chem, D-12489 Berlin, Germany. Additional authors for this research include G.J.V. van Noort and O. Seitz (see also DNA Research).
Keywords for this news article include: Berlin, Europe, Germany, DNA Research, Quantum Dots, Nanotechnology, Emerging Technologies
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