By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on Macromolecular Assemblies have been presented. According to news reporting out of Chapel Hill, North Carolina, by NewsRx editors, research stated, "Nearly all cellular processes are enacted by multi-subunit protein complexes, yet the assembly mechanism of most complexes is not well understood. The anthropomorphism 'protein recruitment' that is used to describe the concerted binding of proteins to accomplish a specific function conceals significant uncertainty about the underlying physical phenomena and chemical interactions governing the formation of macromolecular complexes."
Our news journalists obtained a quote from the research from the University of North Carolina, "We address this deficiency by investigating the diffusion dynamics of two RNA polymerase II subunits, Rpb3 and Rpb9, in regions of live Drosophila cell nuclei that are devoid of chromatin binding sites. Using FRAP microscopy, we demonstrate that both unengaged subunits are incorporated into a broad distribution of complexes, with sizes ranging from free (unincorporated) proteins to those that have been predicted for fully assembled gene transcription units. In live cells, Rpb3 exhibits regions of stability at both size extremes connected by a continuous distribution of complexes. Corresponding measurements on cellular extracts reveal a distribution that retains peaks at the extremes but not in between, suggesting that partially assembled complexes are less stable."
According to the news editors, the research concluded: "We propose that the broad distribution of macromolecular species allows for mechanistic flexibility in the assembly of transcription complexes."
For more information on this research see: RNA Polymerase II Subunits Exhibit a Broad Distribution of Macromolecular Assembly States in the Interchromatin Space of Cell Nuclei. Journal of Physical Chemistry B, 2014;118(2):423-433. Journal of Physical Chemistry B can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Journal of Physical Chemistry B - www.pubs.acs.org/journal/jpcbfk)
Our news journalists report that additional information may be obtained by contacting M.A. Tycon, University of North Carolina, Dept. of Chem, Chapel Hill, NC 27599, United States. Additional authors for this research include M.K. Daddysman and C.J. Fecko (see also Macromolecular Assemblies).
Keywords for this news article include: Polymerase, Chapel Hill, United States, North Carolina, Nanotechnology, Emerging Technologies, Enzymes and Coenzymes, North and Central America, Macromolecular Assemblies
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