By a News Reporter-Staff News Editor at Drug Week -- Investigators discuss new findings in Atomic Force Microscopy. According to news reporting from Highland Heights, Kentucky, by NewsRx journalists, research stated, "Dendrimers are highly customizable nanopolymers with qualities that make them ideal for drug delivery. The high binding affinity of biotin/avidin provides a useful approach to fluorescently label synthesized dendrimer-conjugates in cells and tissues."
The news correspondents obtained a quote from the research from Northern Kentucky University, "In addition, biotin may facilitate delivery of dendrimers through the blood-brain barrier (BBB) via carrier-mediated endocytosis. The purpose of this research was to: (1) measure toxicity using lactate dehydrogenase (LDH) assays of generation (G) 4 biotinylated and non-biotinylated poly(amidoamine) (PAMAM) dendrimers in a co-culture model of the BBB, (2) determine distribution of dendrimers in the rat brain, kidney, and liver following systemic administration of dendrimers, and (3) conduct atomic force microscopy (AFM) on rat brain sections following systemic administration of dendrimers. LDH measurements showed that biotinylated dendrimers were toxic to cell co-culture after 48 h of treatment. Distribution studies showed evidence of biotinylated and non-biotinylated PAMAM dendrimers in brain. AFM studies showed evidence of dendrimers only in brain tissue of treated rats. These results indicate that biotinylation does not decrease toxicity associated with PAMAM dendrimers and that biotinylated PAMAM dendrimers distribute in the brain."
According to the news reporters, the research concluded: "Furthermore, this article provides evidence of nanoparticles in brain tissue following systemic administration of nanoparticles supported by both fluorescence microscopy and AFM."
For more information on this research see: Analysis of Biotinylated Generation 4 Poly(amidoamine) (PAMAM) Dendrimer Distribution in the Rat Brain and Toxicity in a Cellular Model of the Blood-Brain Barrier. Molecules, 2013;18(9):11537-11552. Molecules can be contacted at: Mdpi Ag, Postfach, Ch-4005 Basel, Switzerland. (Springer - www.springer.com; Molecules - www.springerlink.com/content/1420-3049/)
Our news journalists report that additional information may be obtained by contacting R. Hemmer, No Kentucky Univ, CINSAM, Highland Hts, KY 41099, United States. Additional authors for this research include A. Hall, R. Spaulding, B. Rossow, M. Hester, M. Caroway, A. Haskamp, S. Wall, H.A. Bullen, C. Morris and K.L. Haik (see also Atomic Force Microscopy).
Keywords for this news article include: Kentucky, Nanoparticle, United States, Nanotechnology, Highland Heights, Emerging Technologies, Atomic Force Microscopy, North and Central America
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