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Researchers from Nanyang Technological University Detail Findings in Biochemistry

February 25, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Biochemistry. According to news reporting originating in Singapore, Singapore, by NewsRx journalists, research stated, "DNA has the capacity to adopt several distinct structural forms, such as duplex and quadruplex helices, which have been implicated in cellular processes and shown to exhibit important functional properties. Quadruplex-duplex hybrids, generated from the juxtaposition of these two structural elements, could find applications in therapeutics and nanotechnology."

The news reporters obtained a quote from the research from Nanyang Technological University, "Here we used NMR and CD spectroscopy to investigate the thermal stability of two classes of quadruplex-duplex hybrids comprising fundamentally distinct modes of duplex and quadruplex connectivity: Construct I involves the coaxial orientation of the duplex and quadruplex helices with continual base stacking across the two components; Construct II involves the orthogonal orientation of the duplex and quadruplex helices with no base stacking between the two components. We have found that for both constructs, the stability of the quadruplex generally increases with the length of the stem-loop incorporated, with respect to quadruplexes comprising nonstructured loops of the same length, which showed a continuous drop in stability with increasing loop length. The stability of these complexes, particularly Construct I, can be substantially influenced by the base-pair steps proximal to the quadruplex-duplex junction. Bulges at the junction are largely detrimental to the adoption of the desired G-quadruplex topology for Construct I but not for Construct II."

According to the news reporters, the research concluded: "These findings should facilitate future design and prediction of quadruplex-duplex hybrids."

For more information on this research see: Thermal Stability of DNA Quadruplex-Duplex Hybrids. Biochemistry, 2014;53(1):247-257. Biochemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Biochemistry - www.pubs.acs.org/journal/bichaw)

Our news correspondents report that additional information may be obtained by contacting K.W. Lim, Nanyang Technological University, Sch Biol Sci, Singapore 637551, Singapore. Additional authors for this research include Z.J. Khong and A.T. Phan (see also Biochemistry).

Keywords for this news article include: Asia, Biochemistry, DNA Research

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Source: Life Science Weekly


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