By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Proteobacteria are discussed in a new report. According to news reporting from Daejeon, South Korea, by NewsRx journalists, research stated, "Mussels attach to virtually all types of inorganic and organic surfaces in aqueous environments, and catecholamines composed of 3,4-dihydroxy-l-phenylalanine (DOPA), lysine, and histidine in mussel adhesive proteins play a key role in the robust adhesion. DOPA is an unusual catecholic amino acid, and its side chain is called catechol."
The news correspondents obtained a quote from the research from the Graduate School of Nanoscience and Technology, "In this study, we displayed the adhesive moiety of DOPA-histidine on Escherichia coli surfaces using outer membrane protein W as an anchoring motif for the first time. Localization of catecholamines on the cell surface was confirmed by Western blot and immunofluorescence microscopy. Furthermore, cell-to-cell cohesion (i.e., cellular aggregation) induced by the displayed catecholamine and synthesis of gold nanoparticles on the cell surface support functional display of adhesive catecholamines. The engineered E. coli exhibited significant adhesion onto various material surfaces, including silica and glass microparticles, gold, titanium, silicon, poly(ethylene terephthalate), poly(urethane), and poly(dimethylsiloxane). The uniqueness of this approach utilizing the engineered sticky E. coli is that no chemistry for cell attachment are necessary, and the ability of spontaneous E. coli attachment allows one to immobilize the cells on challenging material surfaces such as synthetic polymers."
According to the news reporters, the research concluded: "Therefore, we envision that mussel-inspired catecholamine yielded sticky E. coli that can be used as a new type of engineered microbe for various emerging fields, such as whole living cell attachment on versatile material surfaces, cell-to-cell communication systems, and many others."
For more information on this research see: Preparation of sticky Escherichia coli through surface display of an adhesive catecholamine moiety. Applied and Environmental Microbiology, 2014;80(1):43-53. (American Society for Microbiology - www.asm.org; Applied and Environmental Microbiology - aem.asm.org)
Our news journalists report that additional information may be obtained by contacting J.P. Park, Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology, Daejeon, South Korea. Additional authors for this research include M.J. Choi, S.H. Kim, S.H. Lee and H. Lee (see also Proteobacteria).
Keywords for this news article include: Asia, Daejeon, South Korea, Engineering, Catecholamines, Escherichia coli, Enterobacteriaceae, Biogenic Monoamines, Gammaproteobacteria, Gram Negative Bacteria.
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