By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Membrane Proteins. According to news reporting originating from Cochin, India, by NewsRx correspondents, research stated, "To isolate and characterize bacteriocin, BL8, from the bacteria identified as Bacillus licheniformis from marine environment. One-hundred and twelve bacterial isolates from sediment and water samples collected off the coast of Cochin, India, were screened for antibacterial activity."
Our news editors obtained a quote from the research from the Cochin University of Science and Technology, "Strain BTHT8, identified as Bacillus licheniformis, inhibited the growth of Gram-positive test organisms. The active component labelled as bacteriocin BL8 was partially purified by ammonium sulphate fractionation and was subjected to glycine SDS-PAGE. The band exhibiting antimicrobial activity was electroeluted and analysed using MALDI-TOF mass spectrometry, and the molecular mass was determined as 1.4 kDa. N-terminal amino acid sequencing of BL8 gave a 13 amino acid sequence stretch. Bacteriocin BL8 was stable even after boiling at 100 °C for 30 min and over a wide pH range of 1-12. A novel, pH-tolerant and thermostable bacteriocin BL8, active against the tested Gram-positive bacteria, was isolated from Bacillus licheniformis. This study reports a stable, low molecular weight bacteriocin from Bacillus licheniformis."
According to the news editors, the research concluded: "This bacteriocin can be used to address two important applications: as a therapeutic agent and as a biopreservative in food processing industry."
For more information on this research see: Thermostable Bacteriocin BL8 from Bacillus licheniformis isolated from marine sediment. Journal of Applied Microbiology, 2013;114(3):688-94. (Wiley-Blackwell - www.wiley.com/; Journal of Applied Microbiology - onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2672)
The news editors report that additional information may be obtained by contacting S. Smitha, Dept. of Biotechnology, Cochin University of Science and Technology, Cochin, India (see also Membrane Proteins).
Keywords for this news article include: Asia, India, Cochin, Bacteriocins, Membrane Proteins, Bacterial Proteins, Pore Forming Cytotoxic Proteins.
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