News Column

New Supramolecular Research Study Results Reported from National University

February 25, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Supramolecular Research. According to news reporting out of Mexico City, Mexico, by NewsRx editors, research stated, "The branched respiratory chain in mitochondria from the halotolerant yeast Debaryomyces hansenii contains the classical complexes I, II, III and IV plus a cyanide-insensitive, AMP-activated, alternative-oxidase (AOX). Two additional alternative oxidoreductases were found in this organism: an alternative NADH dehydrogenase (NDH2e) and a mitochondrial isoform of glycerol-phosphate dehydrogenase (MitGPDH)."

Our news journalists obtained a quote from the research from National University, "These monomeric enzymes lack proton pump activity. They are located on the outer face of the inner mitochondrial membrane. NDH2e oxidizes exogenous NADH in a rotenone-insensitive, flavone-sensitive, process. AOX seems to be constitutive; nonetheless, most electrons are transferred to the cytochromic pathway. Respiratory supercomplexes containing complexes I, III and IV in different stoichiometries were detected. Dimeric complex V was also detected. In-gel activity of NADH dehydrogenase, mass spectrometry, and cytochrome c oxidase and ATPase activities led to determine the composition of the putative supercomplexes. Molecular weights were estimated by comparison with those from the yeast Y. lipolytica and they were IV2, I-IV, III2-IV4, V2, I-III2, I-III2-IV, I-III2-IV2, I-III2-IV3 and I-III2-IV4. Binding of the alternative enzymes to supercomplexes was not detected."

According to the news editors, the research concluded: "This is the first report on the structure and organization of the mitochondrial respiratory chain from D."

For more information on this research see: The branched mitochondrial respiratory chain from Debaryomyces hansenii: components and supramolecular organization. Biochimica Et Biophysica Acta, 2014;1837(1):73-84 (see also Supramolecular Research).

Our news journalists report that additional information may be obtained by contacting A. Cabrera-Orefice, Dept. of Molecular Genetics, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico. Additional authors for this research include N. Chiquete-Felix, J. Espinasa-Jaramillo, M. Rosas-Lemus, S. Guerrero-Castillo, A. Pena and S. Uribe-Carvajal.

Keywords for this news article include: Mexico City, Dehydrogenase, Nanotechnology, Emerging Technologies, Enzymes and Coenzymes, Supramolecular Research, North and Central America.

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Source: Life Science Weekly

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