By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Life Science Research are presented in a new report. According to news reporting out of Columbia, Missouri, by NewsRx editors, research stated, "Behavioral bioassays were used to isolate compounds from germinating corn roots that elicit a host recognition response (tight-turning behavior) by neonate larvae of the western corn rootworm Diabrotica virgifera virgifera LeConte. When a behaviorally active extract of germinating corn roots was separated into an aqueous partition and a hexane partition, significantly more larvae (P < 0.05) responded to the recombined partitions than to either partition alone, demonstrating that the active material is a blend comprising both polar and nonpolar compounds."
Our news journalists obtained a quote from the research from the University of Missouri, "When the aqueous partition was separated with reverse-phase solid phase extraction, most of the behavioral activity was retained in the 100% water fraction (F-1). Gas chromatography-mass spectrometry analysis determined that F-1 contained a blend of small sugars, diacids, amino acids, and inorganic compounds. The nonpolar partition was separated on a silica column, and the resulting fractions were tested in combination with F-1 from the aqueous separation. More than 70% of larvae responded to the 100% acetone fraction (fraction B) in combination with F-1, and the response to this treatment was significantly higher than responses to the other nonpolar fractions or to F-1 alone."
According to the news editors, the research concluded: "Methyl esterification of fraction B, followed by gas chromatographic fatty acid methyl ester analysis, confirmed that fraction B primarily consisted of lipids containing fatty acyl groups."
For more information on this research see: Isolation and Characterization of Host Recognition Cues in Corn Roots for Larvae of the Western Corn Rootworm (Coleoptera: Chrysomelidae). Journal of Economic Entomology, 2013;106(6):2354-2363. Journal of Economic Entomology can be contacted at: Entomological Soc Amer, 10001 Derekwood Lane, Ste 100, Lanham, MD 20706-4876, USA (see also Life Science Research).
Our news journalists report that additional information may be obtained by contacting E.J. Bernklau, University of Missouri, Dept. of Agriculture ARS, Columbia, MO 65211, United States. Additional authors for this research include B.E. Hibbard and L.B. Bjostad.
Keywords for this news article include: Columbia, Missouri, United States, Life Science Research, North and Central America
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