By a News Reporter-Staff News Editor at Women's Health Weekly -- Fresh data on Oncology are presented in a new report. According to news reporting from Kolkata, India, by NewsRx journalists, research stated, "Metal oxide nanoparticles are well known to generate oxidative stress and deregulate normal cellular activities. Among these, transition metals copper oxide nanoparticles (CuO NPs) are more compelling than others and able to modulate different cellular responses."
The news correspondents obtained a quote from the research from Jadavpur University, "In this work, we have synthesized and characterized CuO NPs by various biophysical methods. These CuO NPs (~30nm) induce autophagy in human breast cancer cell line, MCF7 in a time-and dose-dependent manner. Cellular autophagy was tested by MDC staining, induction of green fluorescent protein-light chain 3 (GFP-LC3B) foci by confocal microscopy, transfection of pBABE-puro mCherry-EGFP-LC3B plasmid and Western blotting of autophagy marker proteins LC3B, beclin1 and ATG5. Further, inhibition of autophagy by 3-MA decreased LD50 doses of CuO NPs. Such cell death was associated with the induction of apoptosis as revealed by FACS analysis, cleavage of PARP, de-phosphorylation of Bad and increased cleavage product of caspase 3. siRNA mediated inhibition of autophagy related gene beclin1 also demonstrated similar results. Finally induction of apoptosis by 3-MA in CuO NP treated cells was observed by TEM. This study indicates that CuO NPs are a potent inducer of autophagy which may be a cellular defense against the CuO NP mediated toxicity and inhibition of autophagy switches the cellular response into apoptosis. A combination of CuO NPs with the autophagy inhibitor is essential to induce apoptosis in breast cancer cells."
According to the news reporters, the research concluded: "CuO NP induced autophagy is a survival strategy of MCF7 cells and inhibition of autophagy renders cellular fate to apoptosis."
For more information on this research see: Interplay between autophagy and apoptosis mediated by copper oxide nanoparticles in human breast cancer cells MCF7. Biochimica Et Biophysica Acta, 2014;1840(1):1-9 (see also Oncology).
Our news journalists report that additional information may be obtained by contacting D. Laha, Dept. of Life Science and Biotechnology, Jadavpur University, Kolkata, 700 032, India. Additional authors for this research include A. Pramanik, J. Maity, A. Mukherjee, P. Pramanik, A. Laskar and P. Karmakar.
Keywords for this news article include: Asia, India, Kolkata, Oncology, Apoptosis, Nanoparticle, Breast Cancer, Nanotechnology, Women's Health, Emerging Technologies.
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