By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Nanoparticles are discussed in a new report. According to news reporting from Oporto, Portugal, by NewsRx journalists, research stated, "Cationic solid lipid nanoparticles (cSLN) are colloidal carriers for genes or drugs, particularly lipophilic drugs. Several reports exist on their high efficiency, but only a few studies report the effect of cSLNs on living cells."
The news correspondents obtained a quote from the research from National Health Institute, "In the present work, internalization, cell viability (alamar blue assay) and genotoxic potential (alkaline comet assay) of three cSLN formulations (A-C) were evaluated in HepG2 and Caco-2 cells. cSLN showed an average hydrodynamic diameter (z-ave) of 141-222nm, zeta-potential of 55.0-72.5mV and polidispersity indices (PdI) of 0.336-0.421. Dispersion in physiological buffers increased z-ave and PdI. 0.01mgml(-1) cSLN unaffected cell viability, but 1.0mgml(-1) significantly decreased it, being cSLN-C (Compritol-based) the most toxic and HepG2 the most affected. DNA damage was not significantly increased by 0.1mgml(-1) cSLN but damage was observed at 1.0mgml(-1) cSLN-C."
According to the news reporters, the research concluded: "Thus, no genotoxicity is to be expected at concentrations that do not reduce cell viability."
For more information on this research see: Comet assay reveals no genotoxicity risk of cationic solid lipid nanoparticles. Journal of Applied Toxicology, 2014;34(4):395-403. Journal of Applied Toxicology can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Journal of Applied Toxicology - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1099-1263)
Our news journalists report that additional information may be obtained by contacting S. Doktorovova, Natl Hlth Inst Dr Ricardo Jorge INSA, P-4000055 Oporto, Portugal. Additional authors for this research include A.M. Silva, I. Gaivao, E.B. Souto, J.P. Teixeira and P. Martins-Lopes (see also Nanoparticles).
Keywords for this news article include: Oporto, Europe, Portugal, Nanotechnology, Emerging Technologies
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