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Investigators at University of Saarland Describe Findings in Anions

February 25, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Anions are presented in a new report. According to news reporting from Saarbrucken, Germany, by NewsRx journalists, research stated, "Calcium phosphate (CaP) is used for in vitro transfection because of low toxicity and simple and low cost synthesis. The transfection results however vary because the precipitation lacks reproducibility and results in polydispersed, agglomerated particles."

The news correspondents obtained a quote from the research from the University of Saarland, "Here a reproducible, one-step procedure for the preparation of amino-modified CaP nanoparticles (NPs) is described using N-(2-aminoethyl)-3-aminopropyltrimethoxysilane as modifying and dispersing agent. The aim was to produce homogeneous, stable CaP NPs, which are loaded with DNA after particle formation. The refined wet-precipitation method yielded NPs with a narrow size distribution (similar to 140 nm) and positive zeta potential at physiological pH. FTIR and Raman spectroscopy analysis verified the aminosilane modification. Interestingly two types of CaP crystalline structures, Brushite and Hydroxyapatite, can be produced depending on the pH and without hydrothermal treatment. Both CaP crystalline phases were characterized using X-ray diffraction (XRD), transmission electron microscopy (TEM), and Brunauer Emmett Teller (BET) analysis. Both showed very low toxicity and enabled reproducible transfection of A549 cells. The higher surface functionalization ' density of Brushite NPs led to superior pDNA condensation capability and higher transfection in lower NP concentration."

According to the news reporters, the research concluded: "The advantages of the improved synthesis are the achievement of stable, crystalline CaP NPs in higher yield and narrow distributed size achieved by agglomeration reduction even without hazardous surfactant."

For more information on this research see: One-Step Synthesis of Nanosized and Stable Amino-Functionalized Calcium Phosphate Particles for DNA Transfection. Chemistry of Materials, 2013;25(18):3667-3674. Chemistry of Materials can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Chemistry of Materials - www.pubs.acs.org/journal/cmatex)

Our news journalists report that additional information may be obtained by contacting B. Mostaghaci, University of Saarland, Dept. of Biopharmaceut & Pharmaceut Technol, D-66123 Saarbrucken, Germany. Additional authors for this research include B. Loretz, R. Haberkorn, G. Kickelbick and C.M. Lehr (see also Anions).

Keywords for this news article include: Europe, Anions, Germany, Saarbrucken, DNA Research, Phosphoric Acids, Calcium Compounds, Calcium Phosphates, Inorganic Chemicals, Phosphorus Compounds

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Source: Life Science Weekly


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