By a News Reporter-Staff News Editor at Science Letter -- Current study results on Enzymes and Coenzymes have been published. According to news reporting originating in Qingdao, People's Republic of China, by NewsRx journalists, research stated, "The microbial cleavage of dimethylsulfoniopropionate (DMSP) generates volatile DMS through the action of DMSP lyases and is important in the global sulfur and carbon cycles. When released into the atmosphere from the oceans, DMS is oxidized, forming cloud condensation nuclei that may influence weather and climate."
The news reporters obtained a quote from the research from the College of Chemistry and Chemical Engineering, "Six different DMSP lyase genes are found in taxonomically diverse microorganisms, and dddQ is among the most abundant in marine metagenomes. Here, we examine the molecular mechanism of DMSP cleavage by the DMSP lyase, DddQ, from Ruegeria lacuscaerulensis ITI_1157. The structures of DddQ bound to an inhibitory molecule 2-(N-morpholino) ethanesulfonic acid and of DddQ inactivated by a Tyr131Ala mutation and bound to DMSP were solved. DddQ adopts a beta-barrel fold structure and contains a Zn2+ ion and six highly conserved hydrophilic residues (Tyr120, His123, His125, Glu129, Tyr131, and His163) in the active site. Mutational and biochemical analyses indicate that these hydrophilic residues are essential to catalysis. In particular, Tyr131 undergoes a conformational change during catalysis, acting as a base to initiate the beta-elimination reaction in DMSP lysis. Moreover, structural analyses and molecular dynamics simulations indicate that two loops over the substrate-binding pocket of DddQ can alternate between 'open' and 'closed' states, serving as a gate for DMSP entry. We also propose a molecular mechanism for DMS production through DMSP cleavage."
According to the news reporters, the research concluded: "Our study provides important insight into the mechanism involved in the conversion of DMSP into DMS, which should lead to a better understanding of this globally important biogeochemical reaction."
For more information on this research see: Molecular insight into bacterial cleavage of oceanic dimethylsulfoniopropionate into dimethyl sulfide. Proceedings of the National Academy of Sciences of the United States of America, 2014;111(3):1026-1031. Proceedings of the National Academy of Sciences of the United States of America can be contacted at: Natl Acad Sciences, 2101 Constitution Ave NW, Washington, DC 20418, USA. (National Academy of Sciences - www.nasonline.org/; Proceedings of the National Academy of Sciences of the United States of America - www.nasonline.org/publications/pnas/)
Our news correspondents report that additional information may be obtained by contacting C.Y. Li, Ocean Univ Qingdao, Coll Chem & Chem Engn, Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, People's Republic of China. Additional authors for this research include T.D. Wei, S.H. Zhang, X.L. Chen, X. Gao, P. Wang, B.B. Xie, H.N. Su, Q.L. Qin, X.Y. Zhang, J. Yu, H.H. Zhang, B.C. Zhou, G.P. Yang and Y.Z. Zhang (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Qingdao, Enzymes and Coenzymes, People's Republic of China
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