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Researchers from National Institute of Advanced Industrial Science and Technology (NIAIST) Report on Findings in Cell Surface Extensions

February 21, 2014



By a News Reporter-Staff News Editor at Science Letter -- Fresh data on Cell Surface Extensions are presented in a new report. According to news reporting originating from Ibaraki, Japan, by NewsRx correspondents, research stated, "Optical microscopy is generally the first choice to observe microbes and cells. However, its resolution is not always sufficient to reveal specific target structures, such as flagella and pili, which are only nanometers wide."

Our news editors obtained a quote from the research from the National Institute of Advanced Industrial Science and Technology (NIAIST), "ASEM is an attractive higher resolution alternative, as the sample is observed in aqueous solution at atmospheric pressure. Sample pretreatment for ASEM only comprises simple tasks including fixation, gold labeling, and reagent exchange, taking less than 1 h in total. The lengthy sample pretreatments often required for more classical electron microscopies, such as embedding and dehydration, are unnecessary, and native morphology is preserved. In this study, positively charged nanogold particles were used to label the surfaces of bacteria and cultured animal cells, exploiting their net negative surface charge. After gold enhancement to increase the size of the nanogold particles, ASEM imaging of the bacteria in aqueous solution revealed pili and delicate spiral flagella. This natural shape contrasts starkly with images of dried flagella recorded by standard SEM. Positively charged nanogold labeled the plasma membrane of cultured COS7 cells, and after enhancement allowed filopodia as thin as 100 nm in diameter to be clearly visualized."

According to the news editors, the research concluded: "Based on these studies, ASEM combined with positively charged nanogold labeling promises to become an important tool for the study of cell morphology and dynamics in the near future. Microsc. Res. Tech. 77:153-160, 2014."

For more information on this research see: Positively Charged Nanogold Label Allows the Observation of Fine Cell Filopodia and Flagella in Solution by Atmospheric Scanning Electron Microscopy. Microscopy Research and Technique, 2014;77(2):153-160. Microscopy Research and Technique can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Microscopy Research and Technique - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0029)

The news editors report that additional information may be obtained by contacting H. Nishiyama, Natl Inst Adv Ind Sci & Technol, Biomed Res Inst, Tsukuba, Ibaraki 3058566, Japan. Additional authors for this research include K. Teramoto, M. Suga and C. Sato (see also Cell Surface Extensions).

Keywords for this news article include: Asia, Japan, Ibaraki, Flagella, Cell Surface Extensions

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Source: Science Letter


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