By a News Reporter-Staff News Editor at Science Letter -- Investigators publish new report on Cellular Structures. According to news originating from Tokyo, Japan, by NewsRx correspondents, research stated, "To enable the visualization of the distribution and dynamics of intracellular biomolecules and thereby understand the mechanisms of intracellular bioreactions, we developed a specific functional nanoprobe through the combination of a well-designed, cytocompatible phospholipid polymer and molecular beacons (MBs). A water-soluble, amphiphilic phospholipid polymer, poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)-co-N-succinimidyloxycarbonyl tetra(ethylene glycol) methacrylate] (PMBS), was synthesized and conjugated with MBs to form nanoprobes via a chemical reaction between the ester group of N-hydroxysuccinimide and the amine group of the MBs."
Our news journalists obtained a quote from the research from the University of Tokyo, "Surface tension measurements indicated that the polymeric nanoprobes had different conformations in aqueous solution, specifically at a concentration of 1.0 mg/mL. The PMBS, containing the large, hydrophobic BMA, formed polymer aggregates. The carcinoma cells used to test the probes remained 100% viable after incubation with PMBS-MB probes. The polymeric nanoprobes demonstrated not only a high target specificity but also resistance to nonspecific adsorption of proteins compared with unconjugated MBs and were able to penetrate the cytoplasm of the cells, allowing the live imaging of mRNA."
According to the news editors, the research concluded: "In summary, MPG polymer-MB nanoprobes have great potential for practical application for the noninvasive monitoring of intracellular biomolecules and bioreactions in real time."
For more information on this research see: Cell-Membrane-Permeable and Cytocompatible Phospholipid Polymer Nanoprobes Conjugated with Molecular Beacons. Biomacromolecules, 2014;15(1):150-157. Biomacromolecules can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Biomacromolecules - www.pubs.acs.org/journal/bomaf6)
The news correspondents report that additional information may be obtained from X.J. Lin, University of Tokyo, Sch Engn, Dept. of Bioengn, Bunkyo Ku, Tokyo 1138656, Japan. Additional authors for this research include T. Konno and K. Ishihara (see also Cellular Structures).
Keywords for this news article include: Asia, Tokyo, Japan, Cell Membrane, Cellular Structures
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