Singleton has first-hand experience of working with the rapper and actor after he starred in his 1993 film "Poetic Justice" with Janet Jackson.
"Tupac was the guy who I planned to do a lifetime of films with," Singleton said. "His passing deeply affected my life as well as countless people in this world. His life story is as important to my generation."
The Hollywood Reporter said the "Boyz N The Hood" director had been involved with the film two years ago but a deal was never reached.
But now he is said to be rewriting the screenplay before directing and producing the film which could start shooting in the summer.
It is not yet known who will play Shakur, who was shot dead in Las Vegas in 1996.
Shakur's mother Afeni Shakur will be an executive producer on the film.
In January it was announced that a Tupac Shakur musical, "Holler If Ya Hear Me," will open on Broadway in June.
(c) Sky News 2014
Original headline: John Singleton To Direct Tupac Shakur Biopic
Most Popular Stories
- Chobani Counters Competition With Expanded Lineup
- Reid: Bundy Backers Are 'Domestic Terrorists'
- Ex-BP Employee Settles Insider Trading Charges
- Venture Investments in U.S. Highest Since 2001
- Colo. Cleantech Program Calls for Entrepreneurs
- Unemployment Rates Down, Job Gains Up in March
- Hiring Fair for Veterans, Job Seekers
- Recordings Reveal a Not-So-Nice Martinez: Editorial
- VW Beetle Marks 65th Year in U.S.
- The Biebs Crashes Drake's Release Party