By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Investigators publish new report on Biotechnology. According to news reporting from Houston, Texas, by NewsRx journalists, research stated, "The development of efficient and biocompatible non-viral vectors for gene therapy remains a great challenge, and exploiting the properties of both nanoparticle carriers and cationic polymers is an attractive approach. In this work, we have developed gold nanoparticle (AuNP) polyamidoamine (PAMAM) conjugates for use as non-viral transfection agents."
The news correspondents obtained a quote from the research from Rice University, "AuPAMAM conjugates were prepared by crosslinking PAMAM dendrimers to carboxylic-terminated AuNPs via EDC and sulfo-NHS chemistry. EDC and sulfo-NHS have been utilized widely and in numerous applications such as amino acid coupling; however, their use in the coupling of PAMAM dendrimers to AuNPs presents new challenges to form effective and stable constructs for delivery that have not yet been examined. Enhanced colloidal stability and DNA condensation ability was established by probing two critical synthetic parameters: the reaction rate of the PAMAM crosslinking step, and the amine to carboxyl ratio. Based on this work, increasing the amine to carboxyl ratio during conjugation of PAMAM onto AuNPs yielded the optimal vector with respect to colloidal stability and transfection efficiency in vitro."
According to the news reporters, the research concluded: "AuPAMAM conjugates present attractive candidates for non-viral gene delivery due to their commercial availability, ease of fabrication and scale-up, high yield, high transfection efficiency and low cytotoxicity."
For more information on this research see: Optimization of PAMAM-gold nanoparticle conjugation for gene therapy. Biomaterials, 2014;35(5):1725-34. (Elsevier - www.elsevier.com; Biomaterials - www.elsevier.com/wps/product/cws_home/30392)
Our news journalists report that additional information may be obtained by contacting E.R. Figueroa, Rice University, Dept. of Bioengineering, 6500 Main St, Houston, TX 77030, United States. Additional authors for this research include A.Y. Lin, J. Yan, L. Luo, A.E. Foster and R.A Drezek (see also technology.html">Biotechnology).
Keywords for this news article include: Biotechnology, Texas, Houston, Gene Therapy, United States, Bioengineering, Nanotechnology, Gold Nanoparticles, Emerging Technologies, North and Central America.
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