Our news journalists obtained a quote from the research from IPHT, "However, labeling of pathogens such as S. aureus with green fluorescent protein, for example, or the generation of a reliable antibody, which are prerequisites for the application of reproducible isolation techniques, does not always succeed. Here, we present a universal approach for monitoring pathogen traffic after internalization into host cells by fluorescence microscopy and for isolation of bacteria from host-pathogen interaction assays using gold or ferric oxide-core, poly(vinyl alcohol) coated, and fluorescence-labeled nanoparticles (NP). The incubation of S. aureus HG001 with those NP had only minor effects on the bacterial growth in vitro. Quantitative proteome analysis after 24 h of NP incubation revealed that presence of NP provoked only marginal changes in the proteome pattern."
According to the news editors, the research concluded: "The method presented enabled us to investigate the behavior of S. aureus HG001 during infection of S9 human epithelial cells by means of fluorescence microscopy and proteomics using magnetic separation or cell sorting."
For more information on this research see: Labeling of the Pathogenic Bacterium Staphylococcus aureus with Gold or Ferric Oxide-Core Nanoparticles Highlights New Capabilities for Investigation of Host-Pathogen Interactions. Cytometry Part A, 2014;85(2):140-150. Cytometry Part A can be contacted at: Wiley-Blackwell,
The news correspondents report that additional information may be obtained from
Keywords for this news article include: Jena,
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC
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