By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Immunology. According to news reporting originating in Changsha, People's Republic of China, by NewsRx journalists, research stated, "We report the development of a novel single-step, multiplexed, homogeneous immunoassay platform for sensitive detection of protein targets based on our realization of high surface-enhanced raman spectroscopy (SERS) signal enhancement by controlled assembly of SERS nanoparticles. An essential design of this platform is the use of gold nanoparticles or nanorods codecorated with specially reduced antibody half-fragments, nonfluorescent Raman-active dyes, and passivating proteins as the SERS nanoparticles."
The news reporters obtained a quote from the research from Hunan University, "These nanoparticles offer a facile approach to accomplish orientational immobilization of antibodies, minimized interparticle distance, multicolor Raman fingerprint coding, low fluorescence background, as well as excellent biocompatibility and stability. Through sandwiched antibody-antigen interactions, controlled assembly of SERS nanoparticles is realized with a strong SEAS signal achieved via plasmonic coupling, creating an immunoassay platform for rapid, sensitive, multiplexed quantification of proteins. This platform is demonstrated for reproducible quantification of three cytokines, interferon gamma, interleukin-2, and tumor necrosis factor alpha, with large signal-to-noise ratio. It is also successfully applied to multiplexed cytokine analysis for T cell secretion studies in complicated biological samples."
According to the news reporters, the research concluded: "The developed SERS immunoassay platform may create a simple but valuable tool for facilitating accurate validation and early detection of disease biomarkers as well as for point-of-care tests in clinical diagnostics."
For more information on this research see: Surface-Enhanced Raman Spectroscopy-Based, Homogeneous, Multiplexed Immunoassay with Antibody-Fragments-Decorated Gold Nanoparticles. Analytical Chemistry, 2013;85(19):9213-9220. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
Our news correspondents report that additional information may be obtained by contacting Y. Wang, Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo BioSensing & Chemometr, Changsha 410082, Hunan, People's Republic of China. Additional authors for this research include L.J. Tang and J.H. Jiang (see also Immunology).
Keywords for this news article include: Asia, Antibodies, Changsha, Immunology, Nanoparticle, Blood Proteins, Nanotechnology, Immunoglobulins, Emerging Technologies, People's Republic of China
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