By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on Immunology are presented in a new report. According to news reporting out of Helsinki, Finland, by NewsRx editors, research stated, "Nanoparticles have successfully been employed in immunometric assays that require high sensitivity. Certain analytes, however, require dynamic ranges (DRs) around a predetermined cut-off value."
Our news journalists obtained a quote from the research from the University of Helsinki Central Hospital, "Here, we have studied the effects that antibody orientation and addition of free solid-phase and detection antibodies have on assay sensitivity and DR in traditional sandwich-type immunoassays. D-dimer and cardiac troponin I (cTnI), both routinely used in critical care testing, were applied as model analytes. The assays were performed in microtitration wells with preimmobilized solid-phase antibody. Inherently fluorescent nanoparticles coated with second antibody were used to detect the analyte. The selection of antibody orientation and addition of free solid-phase or detection antibody, with nanoparticles and calibrator, desensitized the assays and extended the DR. With D-dimer the upper limit of the DR was improved from 50 to 10,000 ng/ml, and with cTnI from 25 to 1000 ng/ml. Regression analysis with the Stago STA Liatest D-dimer assay yielded a slope (95% confidence interval) of 0.09 (0.07-0.11) and a yintercept of 7.79 (-17.87-2.29) ng/L (n = 65, r = 0.906)."
According to the news editors, the research concluded: "Thus it is concluded that Europium(III)-chelate-doped nanoparticles can also be employed in immunoassays that require wide DRs around a certain cut-off limit."
For more information on this research see: Extension of dynamic range of sensitive nanoparticle-based immunoassays. Analytical Biochemistry, 2014;446():82-86. Analytical Biochemistry can be contacted at: Academic Press Inc Elsevier Science, 525 B St, Ste 1900, San Diego, CA 92101-4495, USA. (Elsevier - www.elsevier.com; Analytical Biochemistry - www.elsevier.com/wps/product/cws_home/622781)
Our news journalists report that additional information may be obtained by contacting H. Hyytia, Helsinki Univ Hosp, Dept. of Clin Chem, HUSLAB, Hus Helsinki 00029, Finland. Additional authors for this research include N. Ristiniemi, P. Laitinen, T. Lovgren and K. Pettersson (see also Immunology).
Keywords for this news article include: Antibodies, Europe, Finland, Helsinki, Immunology, Nanoparticle, Blood Proteins, Nanotechnology, Immunoglobulins, Emerging Technologies
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