News Column

Researchers' Work from Nanchang University Focuses on Phosphotransferases (Alcohol Group Acceptor)

February 18, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on Enzymes and Coenzymes have been presented. According to news reporting originating in Nanchang, People's Republic of China, by NewsRx journalists, research stated, "A simple and sensitive photoluminescence (PL) assay for the activity of a protein kinase based on the selective aggregation of phosphorylated peptide-graphene quantum dot (GQD) conjugates triggered by Zr4+ ion coordination has been established. With more sophisticated design of the peptide substrate sequences, detecting other enzymes could also be possible."

The news reporters obtained a quote from the research from Nanchang University, "Under optimal conditions, a linear relationship between the decreased PL intensity of peptide-GQD conjugates and the concentration of casein kinase II (CK2) in the range from 0.1 to 1.0 unit mL(-1) with a detection limit of 0.03 unit mL(-1) (3 sigma) was obtained. The EC50 value (i.e., the enzyme concentration producing 50% substrate conversion) for CK2 was evaluated to be 0.34 unit mL-1. The proposed method showed potential applications in kinase inhibitor screening. To demonstrate the potential of this GQD-based platform for screening of kinase inhibitors in real biological systems, the inhibition of CK2 phosphorylation activity by four different inhibitors (ellagic acid, 5,6-dichlorobenzimidazole-l-beta-D-ribofuranoside, emodin, and quercetin) was tested in human serum by comparing signals from samples incubated with the inhibitors against that without any inhibitor. As expected, in the presence of inhibitors, the PL intensity increased with increasing inhibitor efficiency."

According to the news reporters, the research concluded: "The IC50 value (inhibitor concentration producing 50% inhibition) for ellagic acid was estimated to be 0.041 mu M. The developed protocol provides a new and promising tool for the analysis of both the enzyme and its inhibitors with low cost and excellent performance."

For more information on this research see: Using Graphene Quantum Dots as Photoluminescent Probes for Protein Kinase Sensing. Analytical Chemistry, 2013;85(19):9148-9155. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)

Our news correspondents report that additional information may be obtained by contacting Y. Wang, Nanchang University, Dept. of Chem, Nanchang 330031, People's Republic of China. Additional authors for this research include L. Zhang, R.P. Liang, J.M. Bai and J.D. Qiu (see also Enzymes and Coenzymes).

Keywords for this news article include: Asia, Nanchang, Peptides, Proteomics, Quantum Dots, Nanotechnology, Protein Kinases, Quantum Physics, Emerging Technologies, Enzymes and Coenzymes, People's Republic of China, Phosphotransferases (Alcohol Group Acceptor)

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Source: Life Science Weekly


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