News Column

Researchers from University of Gothenburg Report Findings in Scleroproteins

February 18, 2014



By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Proteins are discussed in a new report. According to news reporting originating from Gothenburg, Sweden, by NewsRx correspondents, research stated, "Deamidation of glutamine (Q) and asparagine (N) has been recognized as a marker of degradation and aging in ancient proteins. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to study deamidation in wool textiles, we identified eight peptides from alpha-keratin proteins in sheep wool that could potentially be used to assess the level of degradation."

Our news editors obtained a quote from the research from the University of Gothenburg, "For each chosen peptide, the extent of deamidation was determined by comparing the calculated theoretical distribution with the measured distribution using a genetic algorithm that gives the best fit to the measured distribution. Variations in the levels of deamidation were observed between peptides and in modern wool samples buried for up to 8 years in which deamidation levels were relatively low under short-term burial. In contrast, deamidation was higher in archeological textile fragments from medieval sites ranging from the 9th to 13th century in York (United Kingdom) and Newcastle (United Kingdom) and from the 13th to 16th century in Reykholt (Iceland). Major differences were observed between the British and the Icelandic samples, showing a negative correlation between age of samples and levels of deamidation, but highlighting the effect of local environment. In addition, nanoscale liquid chromatography electrospray ionization tandem mass spectrometry (nanoLC ESI-MS/MS) data indicated that deamidation in wool's alpha-keratin was influenced by primary and higher-order structures."

According to the news editors, the research concluded: "Predominance of deamidation on glutamine rather than asparagine in the archeological samples was attributed to a higher abundance of Q in the alpha-helical core domain of keratins, neighboring residues and steric hindrance preventing deamidation of N."

For more information on this research see: Modeling Deamidation in Sheep alpha-Keratin Peptides and Application to Archeological Wool Textiles. Analytical Chemistry, 2014;86(1):567-575. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)

The news editors report that additional information may be obtained by contacting C. Solazzo, University of Gothenburg, Dept. of Conservat, SE-40530 Gothenburg, Sweden. Additional authors for this research include J. Wilson, J.M. Dyer, S. Clerens, J.E. Plowman, I. von Holstein, P.W. Rogers, E.E. Peacock and M.J. Collins (see also Proteins).

Keywords for this news article include: Sweden, Europe, Keratins, Peptides, Gothenburg, Amino Acids, Scleroproteins

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Source: Life Science Weekly


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