By a News Reporter-Staff News Editor at Biotech Week -- Investigators discuss new findings in Neuraminic Acids. According to news reporting originating in Bratislava, Slovakia, by NewsRx journalists, research stated, "We present here comparison of a build-up of two ultrasensitive lectin biosensors based on 2D or 3D architecture. A 2D lectin biosensor was prepared by a covalent immobilisation of lectin Sambucus nigra agglutinin (SNA) recognising sialic acid directly on a mixed self-assembled monolayer (SAM) on planar gold surfaces."
The news reporters obtained a quote from the research from the Institute of Chemistry, "A 3D biosensor was prepared by covalent immobilisation of SNA lectin on a mixed SAM layer formed on gold nanoparticles. Surface plasmon resonance technique allowed to follow kinetics of a mixed SAM (1: 1 mixture of 11-mercaptoundecanoic acid and 6-mercaptohexanol) formation on a bare gold electrode and on an electrode modified by 5 nm and 20 nm gold nanoparticles (AuNPs). Results from the study revealed that a mixed SAM formation is slower on surfaces with increased curvature, the process of SAM formation on all surfaces is completed within 6 min, but a density of thiols on such surfaces differs significantly. Quartz crystal microbalance experiments showed that a surface density of immobilised lectin of (2.53 +/- 0.01) pmol cm(-2) was higher on planar gold surface compared to the surface modified by 20 nm AuNPs with a surface density of (0.94 +/- 0.01) pmol cm(-2). Even though a larger amount of SNA lectin was immobilised on a surface of the 2D biosensor compared to the 3D biosensor, lectin molecules immobilised on AuNPs were more accessible for its analytes - glycoproteins fetuin and asialofetuin, containing different amount of sialic acid on the protein surface."
According to the news reporters, the research concluded: "Most likely a better accessibility of lectin for its analytes on a 3D surface and proper interfacial properties of a 3D surface are behind unprecedented detection limit down to aM level for the lectin biosensor based on such a nanoscale tuned interface."
For more information on this research see: Comparison of the 2D and 3D Nanostructured Lectin-Based Biosensors for In Situ Detection of Sialic Acid on Glycoproteins. International Journal of Electrochemical Science, 2014;9(2):890-900. International Journal of Electrochemical Science can be contacted at: Esg, Borivoja Stevanovica 25-7, Belgrade, 11000, Serbia (see also Neuraminic Acids).
Our news correspondents report that additional information may be obtained by contacting T. Bertok, Slovak Academy Sci, Center Glyc, Inst Chem, Dept. of Glycobiotechnol, Bratislava 84538, Slovakia. Additional authors for this research include A. Sediva, A. Vikartovska and J. Tkac.
Keywords for this news article include: Europe, Slovakia, Bratislava, Biosensing, Sialic Acids, Hydroxy Acids, Bioengineering, Carboxylic Acids, Neuraminic Acids, Bionanotechnology, Nanobiotechnology, Organic Chemicals, Gold Nanoparticles, Emerging Technologies
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